Wang Bo, Jiang Yufeng, Wang Zhuo, Li Fangfang, Xing Guoqiang, Peng Xinyu, Zhang Shijie, Lv Hailong
Department of Hepatobiliary Surgery, The First Affiliated Hospital, School of Medicine, Shihezi University, Xinjiang, China.
Department of Histology and Embryology, School of Medicine, Shihezi University, Xinjiang, China
Antimicrob Agents Chemother. 2015 Nov;59(11):6946-51. doi: 10.1128/AAC.04340-14. Epub 2015 Aug 31.
Spillage of cyst contents during surgery is the major cause of recurrences of hydatidosis, also called cystic echinococcosis (CE). Currently, many scolicidal agents are used for inactivation of the cyst contents. However, due to complications in the use of those agents, new and more-effective treatment options are urgently needed. The aim of this study was to investigate the in vitro efficacy of arsenic trioxide (ATO) against Echinococcus granulosus protoscolices. Protoscolices of E. granulosus were incubated in vitro with 2, 4, 6, and 8 μmol/liter ATO; viability of protoscolices was assessed daily by microscopic observation of movements and 0.1% eosin staining. A small sample from each culture was processed for scanning and transmission electron microscopy. ATO demonstrated a potent ability to kill protoscolices, suggesting that ATO may represent a new strategy in treating hydatid cyst echinococcosis. However, the in vivo efficacy and possible side effects of ATO need to be explored.
手术过程中囊肿内容物的溢出是包虫病（又称囊性棘球蚴病，CE）复发的主要原因。目前，许多杀头节剂被用于使囊肿内容物失活。然而，由于这些药剂使用过程中的并发症，迫切需要新的、更有效的治疗选择。本研究的目的是调查三氧化二砷（ATO）对细粒棘球绦虫原头节的体外疗效。将细粒棘球绦虫的原头节在体外与2、4、6和8微摩尔/升的ATO一起孵育；通过显微镜观察活动情况和0.1%伊红染色每天评估原头节的活力。对每种培养物的一小部分样本进行扫描和透射电子显微镜检查。ATO显示出强大的杀死原头节的能力，表明ATO可能代表治疗棘球蚴囊肿病的一种新策略。然而，ATO的体内疗效和可能的副作用需要进一步探索。
