Furukawa Hiroshi, Oka Shomi, Shimada Kota, Masuo Kiyoe, Nakajima Fumiaki, Funano Shunichi, Tanaka Yuki, Komiya Akiko, Fukui Naoshi, Sawasaki Tatsuya, Tadokoro Kenji, Nose Masato, Tsuchiya Naoyuki, Tohma Shigeto
Clinical Research Center for Allergy and Rheumatology, Sagamihara Hospital, National Hospital Organization, Sagamihara, Japan. ; Molecular and Genetic Epidemiology Laboratory, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Department of Rheumatology, Sagamihara Hospital, National Hospital Organization, Sagamihara, Japan. ; Department of Rheumatic Diseases, Tokyo Metropolitan Tama Medical Center, Fuchu, Japan.
Biomark Insights. 2015 Aug 16;10:63-73. doi: 10.4137/BMI.S28209. eCollection 2015.
Interstitial lung disease (ILD) is frequently associated with collagen disease. It is then designated as collagen vascular disease-associated ILD (CVD-ILD), and influences patients' prognosis. The prognosis of acute-onset diffuse ILD (AoDILD) occurring in patients with collagen disease is quite poor. Here, we report our investigation of auto-antibody (Ab) profiles to determine whether they may be useful in diagnosing CVD-ILD or AoDILD in collagen disease. Auto-Ab profiles were analyzed using the Lambda Array Beads Multi-Analyte System, granulocyte immunofluorescence test, Proto-Array Human Protein Microarray, AlphaScreen assay, and glutathione S-transferase capture enzyme-linked immunosorbent assay in 34 patients with rheumatoid arthritis (RA) with or without CVD-ILD and in 15 patients with collagen disease with AoDILD. The average anti-major histocompatibility complex class I-related chain A (MICA) Ab levels were higher in RA patients with CVD-ILD than in those without (P = 0.0013). The ratio of the average anti-MICA Ab level to the average anti-human leukocyte antigen class I Ab level (ie, MICA/Class I) was significantly higher in RA patients with CVD-ILD compared with those without (P = 4.47 × 10(-5)). To the best of our knowledge, this is the first report of auto-Ab profiles in CVD-ILD. The MICA/Class I ratio could be a better marker for diagnosing CVD-ILD than KL-6 (Krebs von den lungen-6).
间质性肺疾病（ILD）常与胶原病相关。随后它被指定为胶原血管病相关的ILD（CVD-ILD），并影响患者的预后。胶原病患者中发生的急性起病弥漫性ILD（AoDILD）的预后相当差。在此，我们报告我们对自身抗体（Ab）谱的研究，以确定它们是否可用于诊断胶原病中的CVD-ILD或AoDILD。使用λ阵列磁珠多分析物系统、粒细胞免疫荧光试验、Proto-Array人类蛋白质微阵列、AlphaScreen分析和谷胱甘肽S-转移酶捕获酶联免疫吸附试验，对34例有或无CVD-ILD的类风湿关节炎（RA）患者以及15例有AoDILD的胶原病患者的自身抗体谱进行了分析。有CVD-ILD的RA患者的平均抗主要组织相容性复合体I类相关链A（MICA）抗体水平高于无CVD-ILD的患者（P = 0.0013）。与无CVD-ILD的RA患者相比，有CVD-ILD的RA患者的平均抗MICA抗体水平与平均抗人类白细胞抗原I类抗体水平的比值（即MICA/ I类）显著更高（P = 4.47×10⁻⁵）。据我们所知，这是关于CVD-ILD中自身抗体谱的首次报告。MICA/I类比值可能是比KL-6（克雷布斯冯登肺-6）更好的诊断CVD-ILD的标志物。
