Suzuki K, Tagami K
Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba, Japan.
Int J Sports Med. 2015 Dec;36(14):1186-91. doi: 10.1055/s-0035-1555856. Epub 2015 Sep 2.
Among athletes, Staphylococcus aureus is thought to be transmitted by close physical contact with carriers. Nevertheless, evidence is limited with regard to both the tracking of individual strains and the role of S. aureus on the skin's surface. We investigated its transmission using molecular genotyping and the presence of S. aureus on the skin during exercise. In the first study, nasal samples were obtained from 172 athletes over a period of up to one year. The 200 strains of S. aureus collected from these athletes were genotyped, and transmission of S. aureus was detected by phage open reading frame typing (POT). In the second study, the presence of S. aureus on the skin's surface was compared between nasal carriers (n=9) and non-nasal carriers (n=9), who had participated in the first study. In the first study, 10 cases of transmission were confirmed. In the second study, exercise-induced sweating increased S. aureus isolates from the skin's surface (before vs. after exercise: 5.2±5.4 vs. 41.7±40.6 CFU/ml) in nasal carriers. In 5 of 9 nasal carriers, S. aureus isolates from the skin's surface were clonally identical to those from the nares. These results identify a major route of S. aureus transmission among athletes and provide insight into the role played by exercise-induced sweating in nasal carriers.
在运动员中，金黄色葡萄球菌被认为是通过与携带者的密切身体接触传播的。然而，关于个体菌株的追踪以及金黄色葡萄球菌在皮肤表面的作用，证据有限。我们使用分子基因分型和运动期间皮肤表面金黄色葡萄球菌的存在情况来研究其传播。在第一项研究中，在长达一年的时间里从172名运动员身上采集了鼻腔样本。对从这些运动员身上收集的200株金黄色葡萄球菌进行基因分型，并通过噬菌体开放阅读框分型（POT）检测金黄色葡萄球菌的传播。在第二项研究中，比较了参与第一项研究的鼻腔携带者（n = 9）和非鼻腔携带者（n = 9）皮肤表面金黄色葡萄球菌的存在情况。在第一项研究中，确认了10例传播病例。在第二项研究中，运动引起的出汗增加了鼻腔携带者皮肤表面的金黄色葡萄球菌分离株数量（运动前与运动后：5.2±5.4 vs. 41.7±40.6 CFU/ml）。在9名鼻腔携带者中的5名中，皮肤表面的金黄色葡萄球菌分离株与鼻腔中的分离株克隆相同。这些结果确定了运动员中金黄色葡萄球菌传播的主要途径，并深入了解了运动引起的出汗在鼻腔携带者中所起的作用。
