Toshkova K, Annemüller C, Lämmler C
National Center of Infectious and Parasitic Diseases, Sofia, Bulgaria.
FEMS Microbiol Lett. 2001 Aug 7;202(1):17-24. doi: 10.1111/j.1574-6968.2001.tb10774.x.
The present study was designed to investigate the significance and the relationship between nasal carriage of Staphylococcus aureus and staphylococcal skin infections. Thirty-one S. aureus strains, isolated from 12 patients with chronic and recurrent skin infections, one patient with septicemia and one patient with otitis externa were studied. The staphylococcal strains were isolated from the site of infection and from the anterior nares of each patient. The identity of both strains of each pair could be demonstrated by determination of phenotypic properties and by genotyping of the isolates. The phenotypic properties included hemolytic activities, antibiotic resistance data, and the production of enterotoxins. The identity was additionally confirmed by phage-typing, by determination of the size and the number of repeats of the X region of spa gene, by determination of gene polymorphisms of coa gene and by macrorestriction analysis of the chromosomal DNA of the isolates by pulsed-field gel electrophoresis. The present results showed an identity of the S. aureus obtained from anterior nares and from skin infection of each patient indicating the importance of nasal carriage of these bacteria for development of human skin infection.
本研究旨在调查金黄色葡萄球菌鼻腔携带与葡萄球菌皮肤感染之间的意义及关系。对从12例慢性复发性皮肤感染患者、1例败血症患者和1例外耳道炎患者中分离出的31株金黄色葡萄球菌菌株进行了研究。葡萄球菌菌株从每个患者的感染部位和前鼻孔分离得到。每对菌株的同一性可通过表型特征测定和分离株的基因分型来证明。表型特征包括溶血活性、抗生素耐药数据和肠毒素产生。此外，通过噬菌体分型、spa基因X区域重复序列的大小和数量测定、coa基因的基因多态性测定以及通过脉冲场凝胶电泳对分离株染色体DNA进行宏观限制性分析来确认同一性。目前的结果表明，从每个患者的前鼻孔和皮肤感染中分离出的金黄色葡萄球菌具有同一性，这表明这些细菌的鼻腔携带对于人类皮肤感染的发生具有重要意义。
