Thirunavukarasu K, Purushothaman S, Gowthaman M K, Nakajima-Kambe T, Rose C, Kamini N R
Department of Biomedical Engineering, Sathyabama University, Sholinganallur, Chennai, 600 119 India.
Department of Biotechnology, Central Leather Research Institute, Adyar, Chennai, 600 020 India.
J Food Sci Technol. 2015 Sep;52(9):5772-80. doi: 10.1007/s13197-014-1697-8. Epub 2015 Jan 4.
Fish meal has been used as an additional nitrogen source and fish oil as inducer for the growth and production of lipase from Cryptococcus sp. MTCC 5455. A response surface design illustrated that the optimum factors influencing lipase production were fish meal, 1.5 %, w/v, Na2HPO4, 0.2 %, w/v, yeast extract, 0.25 %, w/v and sardine oil, 2.0 %, w/v with an activity of 71.23 U/mL at 96 h and 25 °C, which was 48.39 % higher than the conventional one-factor-at-a-time method. The crude concentrated enzyme hydrolyzed polyurethane (PUR) efficiently and hydrolysis was 94 % at 30 °C and 96 h. The products, diethylene glycol and adipic acid were quantified by HPLC and scanning electron microscopic studies of the degraded polymer showed significant increase in size of the holes from 24 to 72 h of incubation. Hydrolysis of PUR within 96 h makes the lipase novel for disposal of PUR and provides an innovative solution to the problems created by plastic wastes.
鱼粉已被用作额外的氮源，鱼油则作为诱导剂，用于隐球菌属MTCC 5455脂肪酶的生长和生产。响应面设计表明，影响脂肪酶生产的最佳因素为：1.5%（w/v）的鱼粉、0.2%（w/v）的Na2HPO4、0.25%（w/v）的酵母提取物和2.0%（w/v）的沙丁鱼油，在96小时和25°C条件下，脂肪酶活性为71.23 U/mL，比传统的一次单因素法高出48.39%。粗浓缩酶能有效水解聚氨酯（PUR），在30°C和96小时时水解率为94%。通过高效液相色谱法对产物二甘醇和己二酸进行了定量分析，对降解聚合物的扫描电子显微镜研究表明，在孵育24至72小时期间，孔洞尺寸显著增大。96小时内对PUR的水解使得该脂肪酶成为处理PUR的新型酶，并为塑料废物产生的问题提供了创新解决方案。
