Balli Umut, Aydogdu Ahmet, Dede Figen Ongoz, Turer Cigdem Coskun, Guven Berrak
Department of Periodontology, Faculty of Dentistry, Bulent Ecevit University, Zonguldak, Turkey.
Department of Periodontology, Faculty of Dentistry, Biruni University, Istanbul, Turkey.
J Periodontol. 2015 Dec;86(12):1396-404. doi: 10.1902/jop.2015.150270. Epub 2015 Sep 14.
To investigate changes in the levels and relative ratios of sclerostin, osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand (RANKL) in the gingival crevicular fluid (GCF) of patients with periodontitis after non-surgical periodontal treatment.
Fifty-four individuals (27 healthy controls and 27 patients with chronic periodontitis [CP]) were enrolled in the study. Periodontitis patients received non-surgical periodontal therapy. GCF sampling and clinical periodontal parameters were assessed before and 6 weeks after therapy. Sclerostin, OPG, and RANKL levels were measured by enzyme-linked immunosorbent assay, and their relative ratios were calculated.
Total amounts and concentrations of sclerostin were significantly higher in patients with CP than in healthy individuals (P <0.025) and decreased after treatment (P <0.05). The RANKL/OPG ratio was significantly lower in healthy individuals than in patients with periodontitis before and after treatment (P <0.025), but no significant difference was observed in patients with periodontitis after treatment (P >0.05). The sclerostin/OPG and sclerostin/RANKL ratios were significantly lower in healthy individuals than in patients with periodontitis before and after treatment (P <0.025) and decreased in patients with periodontitis after treatment (P <0.05).
The GCF sclerostin level may be more reliable than the RANKL/OPG ratio as a diagnostic and prognostic marker of periodontal disease and treatment outcome. Regulation of sclerostin levels may aid the development of new therapeutic strategies for the treatment of periodontal disease.
研究非手术牙周治疗后牙周炎患者龈沟液（GCF）中硬化蛋白、骨保护素（OPG）和核因子κB受体激活剂配体（RANKL）的水平及相对比值的变化。
本研究纳入54例个体（27例健康对照者和27例慢性牙周炎[CP]患者）。牙周炎患者接受非手术牙周治疗。在治疗前和治疗后6周评估龈沟液采样和临床牙周参数。通过酶联免疫吸附测定法测量硬化蛋白、OPG和RANKL水平，并计算其相对比值。
CP患者中硬化蛋白的总量和浓度显著高于健康个体（P<0.025），治疗后降低（P<0.05）。健康个体的RANKL/OPG比值在治疗前后均显著低于牙周炎患者（P<0.025），但牙周炎患者治疗后未观察到显著差异（P>0.05）。健康个体的硬化蛋白/OPG和硬化蛋白/RANKL比值在治疗前后均显著低于牙周炎患者（P<0.025），牙周炎患者治疗后降低（P<0.05）。
作为牙周疾病和治疗结果的诊断及预后标志物，龈沟液硬化蛋白水平可能比RANKL/OPG比值更可靠。调节硬化蛋白水平可能有助于开发治疗牙周疾病的新治疗策略。
