中国荷斯坦奶牛全基因组关联研究揭示了两个与体细胞评分相关的候选基因，体细胞评分可作为乳腺炎易感性的指标。
Genome-wide association study in Chinese Holstein cows reveal two candidate genes for somatic cell score as an indicator for mastitis susceptibility.
作者信息
Wang Xiao, Ma Peipei, Liu Jianfeng, Zhang Qin, Zhang Yuan, Ding Xiangdong, Jiang Li, Wang Yachun, Zhang Yi, Sun Dongxiao, Zhang Shengli, Su Guosheng, Yu Ying
机构信息
Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture of China, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, People's Republic of China.
Department of Molecular Biology and Genetics, Center for Quantitative Genetics and Genomics, Aarhus University, DK-8830, Tjele, Denmark.
出版信息
BMC Genet. 2015 Sep 15;16:111. doi: 10.1186/s12863-015-0263-3.
BACKGROUNDS
Bovine mastitis is a typical inflammatory disease causing seriously economic loss. Genome-wide association study (GWAS) can be a powerful method to promote marker assistant selection of this kind of complex disease. The present study aimed to analyze and identify single nucleotide polymorphisms (SNPs) and candidate genes that associated with mastitis susceptibility traits in Chinese Holstein.
RESULTS
Forty eight SNPs were identified significantly associated with mastitis resistance traits in Chinese Holstein cows, which are mainly located on the BTA 14. A total of 41 significant SNPs were linked to 31 annotated bovine genes. Gene Ontology and pathway enrichment revealed 5 genes involved in 32 pathways, in which, TRAPPC9 and ARHGAP39 genes participate cell differentiation and developmental pathway together. The six common genome-wide significant SNPs are found located within TRAPPC9 and flanking ARHGAP39 genes.
CONCLUSIONS
Our data identified the six SNPs significantly associated with SCS EBVs, which suggest that their linked two genes (TRAPPC9 and ARHGAP39) are novel candidate genes of mastitis susceptibility in Holsteins.
背景
奶牛乳腺炎是一种典型的炎症性疾病，会造成严重的经济损失。全基因组关联研究（GWAS）是促进此类复杂疾病标记辅助选择的有力方法。本研究旨在分析和鉴定与中国荷斯坦奶牛乳腺炎易感性性状相关的单核苷酸多态性（SNP）和候选基因。
结果
在中国荷斯坦奶牛中鉴定出48个与乳腺炎抗性性状显著相关的SNP，主要位于牛14号染色体上。共有41个显著的SNP与31个已注释的牛基因连锁。基因本体论和通路富集分析显示5个基因参与32条通路，其中，TRAPPC9和ARHGAP39基因共同参与细胞分化和发育通路。发现6个常见的全基因组显著SNP位于TRAPPC9基因内和ARHGAP39基因侧翼。
结论
我们的数据鉴定出6个与体细胞评分（SCS）估计育种值显著相关的SNP，这表明与其连锁的两个基因（TRAPPC9和ARHGAP39）是荷斯坦奶牛乳腺炎易感性的新候选基因。
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