ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, E-18071 Granada, Spain.
ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, E-18071 Granada, Spain.
Biosens Bioelectron. 2016 Mar 15;77:51-5. doi: 10.1016/j.bios.2015.09.012. Epub 2015 Sep 8.
This work reports on the development of a 3D microfluidic paper-based device (3D µPAD) for glucose detection using organic-inorganic hybrid nanoflower technology to immobilize the bi-enzymatic system (glucose oxidase and horseradish peroxidase). The system is based on nanoflowerssupported on cellulose paper (the microreactor zone) coupled to 3,3',5,5'-tetramethylbenzidine (TMB) as the colorimetric probe in the detection zone. We used a digital camera for the quantitative analysis of glucose with the S coordinate of the HSV color space as the analytical parameter. Under optimal operational conditions, linearity was observed for glucose concentrations up to 300 μM, with a detection limit of 15.6 µM. The biosensor is reusable and remains stable for 75 days in conventional storage conditions.
本工作报道了一种使用有机-无机杂化纳米花技术固定双酶系统（葡萄糖氧化酶和辣根过氧化物酶）的 3D 微流控纸基器件（3D µPAD）用于葡萄糖检测的研究。该系统基于纳米花支撑在纤维素纸上（微反应区），并与 3,3',5,5'-四甲基联苯胺（TMB）作为检测区中的比色探针。我们使用数码相机对葡萄糖进行定量分析，以 HSV 颜色空间的 S 坐标作为分析参数。在最佳操作条件下，观察到葡萄糖浓度高达 300 μM 的线性范围，检测限为 15.6 μM。该生物传感器可重复使用，并在常规储存条件下稳定 75 天。
