Shakya Anshul, Singh Gireesh Kumar, Chatterjee Shyam Sunder, Kumar Vikas
Department of Pharmaceutics, Neuropharmacology Research Laboratory, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
(Retired) Head of the Pharmacological Research Laboratories, Dr. Willmar Schwabe GmbH & Co. KG, Karlsruhe, Germany.
J Intercult Ethnopharmacol. 2014 Oct-Dec;3(4):173-8. doi: 10.5455/jice.20140912021115. Epub 2014 Sep 22.
The aim was to test whether the ethanolic extract of Fumaria indica (FI) possesses anti-inflammatory and analgesic activities, and fumaric acid (FA) could be one of its bioactive constituent involved in such activities of the extract.
For anti-inflammatory activity, carrageenan-induced edema and cotton pellet induced granuloma tests in rats and for analgesic activity rat tail flick test and hot plate and acetic acid writhing tests in mice were used. All tests were performed after seven daily oral doses of the FI extract (100, 200, and 400 mg/kg/day) and pure FA (1.25, 2.50, and 5.00 mg/kg/day).
Anti-inflammatory activities of FI and FA were observed in carrageenan-induced edema and cotton pallet granuloma even after their lowest tested doses. No analgesic activity of lowest tested dose of FA was observed in the acetic acid writhing test, but likewise, all tested dose levels of FI, higher tested dose levels of FA were also possess significant analgesic activity in this test. Further, significant analgesic activities of both FI and FA in hot plate and tale flick tests were observed after all their tested doses.
These observations are in agreement with our working hypothesis on the connection of FA in mode(s) of action(s) of FI, and reaffirm the conviction that FI could be an herbal alternative against fibromyalgia and other pathologies often associate with, or caused by, inflammatory processes.
本研究旨在测试印度紫堇乙醇提取物（FI）是否具有抗炎和镇痛活性，以及富马酸（FA）是否为该提取物此类活性的生物活性成分之一。
通过角叉菜胶诱导大鼠足跖肿胀和棉球诱导大鼠肉芽肿试验评估抗炎活性，通过小鼠甩尾试验、热板试验和醋酸扭体试验评估镇痛活性。在连续7天每日口服FI提取物（100、200和400 mg/kg/天）和纯FA（1.25、2.50和5.00 mg/kg/天）后进行所有试验。
即使在最低测试剂量下，FI和FA对角叉菜胶诱导的足跖肿胀和棉球肉芽肿均具有抗炎活性。在醋酸扭体试验中，未观察到最低测试剂量的FA具有镇痛活性，但同样地，FI的所有测试剂量水平、FA的较高测试剂量水平在此试验中也具有显著的镇痛活性。此外，在热板试验和甩尾试验中，FI和FA的所有测试剂量均具有显著的镇痛活性。
这些观察结果与我们关于FA在FI作用方式中所起作用的工作假设一致，并再次证实FI可能是一种治疗纤维肌痛及其他常与炎症过程相关或由炎症过程引起的疾病的草药替代品。
