通过使用“Mucap试验”从禽肉产品中快速鉴定沙门氏菌。

Rapid identification of Salmonella from poultry meat products by using 'Mucap Test'.

作者信息

Humbert F, Salvat G, Colin P, Lahellec C, Bennejean G

机构信息

C.N.E.V.A., Station Expérimentale d'Aviculture, Ploufragan, France.

出版信息

Int J Food Microbiol. 1989 Feb;8(1):79-83. doi: 10.1016/0168-1605(89)90083-4.

DOI:10.1016/0168-1605(89)90083-4

PMID:2641489

Abstract

A study was made in order to improve a new Salmonella identification test (Mucap Test) in which umbelliferone is released, giving a blue fluorescent light under a Wood lamp, after contact with Salmonella colonies. The study concerned 354 colonies, previously isolated from 55 poultry meat samples. Two enrichment media [Tetrathionate Bile Broth (TBB) and Rappaport Vassiliadis (RV)] and two isolation media [Brilliant Green Agar (BGA) and Desoxycholate Agar (DA)] were used, and the results of the test obtained respectively with each association were compared. The sensitivity was consistently good, but the specificity of the test was generally poor. The best association seemed to be RV/DA which gave 85% specificity, against 39% for TBB/BGA, 58% for TBB/DA, and 77% for RV/BGA. The predominant genera responsible for false-positive results were Pseudomonas and Proteus Providencia.

摘要

为改进一种新的沙门氏菌鉴定试验（Mucap试验）开展了一项研究。在该试验中，伞形酮在与沙门氏菌菌落接触后会释放出来，在伍德灯下发出蓝色荧光。该研究涉及先前从55份禽肉样本中分离出的354个菌落。使用了两种增菌培养基[四硫磺酸盐胆盐肉汤（TBB）和Rappaport Vassiliadis（RV）]以及两种分离培养基[亮绿琼脂（BGA）和脱氧胆酸盐琼脂（DA）]，并比较了分别使用每种组合所获得的试验结果。该试验的敏感性一直良好，但特异性通常较差。最佳组合似乎是RV/DA，其特异性为85%，而TBB/BGA为39%，TBB/DA为58%，RV/BGA为77%。导致假阳性结果的主要菌属是假单胞菌属和普罗威登斯变形杆菌。