Kumar Sunil, Sharma Gaurav, Sidiq Tabasum, Khajuria Anamika, Jain Mahendra, Bhagwat Deepak, Dhar K L
Department of Pharmacology, School of Pharmaceutical Sciences Shoolini University Solan (H.P.) 173229, India.
Division of Pharmacology, Indian Institute of Integrative Medicine (CSIR), Jammu 180001, India.
EXCLI J. 2014 Feb 17;13:137-50. eCollection 2014.
In order to evaluate the role of ethyl acetate fraction (PB-EtAC) obtained from the Phyllostachys bambusoides leaves in the modulation of immune responses, detailed studies were carried out using a panel of in vivo assays. Oral administration of PB-EtAC (50-200 mg/Kg) stimulated the IgM and IgG titre expressed in the form of haemagglutination antibody (HA) titre. Further, it elicited a dose related increase in the delayed type hypersensitivity reaction (DTH) after 24 and 48 h in BALB/c mice. Besides augmenting the humoral and cell mediated immune response, the concentration of cytokines (IFN-γ, IL-2, and IL-4) in serum with respect to T cell interactions also increased significantly. It also induced macrophage phagocytosis, and nitric oxide (NO) production which resulted in a high degree of protection against Candida albicans and carbon clearance. Moreover, the enhancement in CD4 and CD8 cell populations as revealed by flow cytometry. Taken together this in vivo and ex vivo preclinical data, our results suggested that PB-EtAC acts as an effective immunostimulator eliciting both Th1 and Th2 immune responses. We are reporting first time the immunostimulatory potential of P. bambusoides and it might be regarded as a biological response modifier.
为了评估从毛竹叶片中获得的乙酸乙酯部位（PB-EtAC）在调节免疫反应中的作用，我们使用了一系列体内试验进行了详细研究。口服PB-EtAC（50-200mg/Kg）可刺激以血凝抗体（HA）滴度形式表达的IgM和IgG滴度。此外，在BALB/c小鼠中，24小时和48小时后，它引起了迟发型超敏反应（DTH）的剂量相关增加。除了增强体液免疫和细胞介导的免疫反应外，血清中与T细胞相互作用相关的细胞因子（IFN-γ、IL-2和IL-4）浓度也显著增加。它还诱导巨噬细胞吞噬作用和一氧化氮（NO）产生，从而对白色念珠菌和碳清除具有高度保护作用。此外，流式细胞术显示CD4和CD8细胞群体增加。综合这些体内和体外临床前数据，我们的结果表明PB-EtAC作为一种有效的免疫刺激剂，可引发Th1和Th2免疫反应。我们首次报道了毛竹的免疫刺激潜力，它可能被视为一种生物反应调节剂。
