Dos Santos Matheus M, Olaleye Mary T, Ineu Rafael P, Boligon Aline A, Athayde Margareth L, Barbosa Nilda Bv, Rocha João Batista Teixeira
Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil.
Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
EXCLI J. 2014 Mar 31;13:323-30. eCollection 2014.
Ethnobotanical claims regarding Kigelia africana reported antiulcer properties as part of its medicinal application. In this work, aqueous leaf extract from K. africana was investigated for its phytochemical constituents and antiulcer potential against ethanol-induced ulcer in rats. The participation of oxidative stress on ethanol-induced ulcer and the potential protective antioxidant activity of K. africana extracts were investigated by determining vitamin C and thiobarbituric acid reactive species (TBARS) contents in the gastric mucosa of rats. The HPLC analysis showed the presence of gallic acid, chlorogenic acid, caffeic acid and also the flavonoids rutin, quercetin and kaempferol in the aqueous plant extract. Oral treatment with K. africana extract (1.75; 3.5; 7 and 14 mg/kg) one hour after ulcer induction with ethanol decreased in a dose dependent manner the ulcer index. Ethanol increased significantly stomachal TBARS levels and decreased vitamin C content when compared to the control animals. K. africana blunted the ethanol-induced oxidative stress and restored vitamin C content to the control levels. The present results indicate that the aqueous leaf extract from K. africana possesses antiulcer potential. The presence of flavonoids in plant extract suggests that its antiulcerogenic potential is associated with antioxidant activity. Of particular therapeutic potential, K. africana was effective against ethanol even after the induction of ulcer, indicating that it can have protective and curative effects against gastric lesion.
关于非洲吊灯树的民族植物学研究称，其具有抗溃疡特性，这是其药用价值的一部分。在本研究中，对非洲吊灯树的叶水提取物进行了植物化学成分分析，并研究了其对大鼠乙醇诱导型溃疡的抗溃疡潜力。通过测定大鼠胃黏膜中维生素C和硫代巴比妥酸反应性物质（TBARS）的含量，研究了氧化应激在乙醇诱导型溃疡中的作用以及非洲吊灯树提取物潜在的抗氧化保护活性。高效液相色谱分析表明，该植物水提取物中存在没食子酸、绿原酸、咖啡酸以及黄酮类化合物芦丁、槲皮素和山奈酚。在乙醇诱导溃疡1小时后，口服非洲吊灯树叶提取物（1.75；3.5；7和14毫克/千克），溃疡指数呈剂量依赖性降低。与对照动物相比，乙醇显著提高了胃中TBARS水平并降低了维生素C含量。非洲吊灯树提取物减轻了乙醇诱导的氧化应激，并将维生素C含量恢复到对照水平。目前的研究结果表明，非洲吊灯树叶水提取物具有抗溃疡潜力。植物提取物中黄酮类化合物的存在表明其抗溃疡潜力与抗氧化活性有关。特别具有治疗潜力的是，非洲吊灯树即使在溃疡诱导后对乙醇仍有效果，这表明它对胃部病变具有保护和治疗作用。
