Lin Xing, Wu Jiamin, Zheng Guoan, Dai Qionghai
Department of Automation, Tsinghua University, Beijing, 100084, China ; Beijing Key Laboratory of Multi-dimension & Multi-scale Computational Photography (MMCP), Tsinghua University, Beijing, 100084, China.
Biomedical Engineering & Electrical Engineering, University of Connecticut, Storrs, Connecticut, 06269, USA.
Biomed Opt Express. 2015 Aug 3;6(9):3179-89. doi: 10.1364/BOE.6.003179. eCollection 2015 Sep 1.
This paper proposes a novel approach for high-resolution light field microscopy imaging by using a camera array. In this approach, we apply a two-stage relay system for expanding the aperture plane of the microscope into the size of an imaging lens array, and utilize a sensor array for acquiring different sub-apertures images formed by corresponding imaging lenses. By combining the rectified and synchronized images from 5 × 5 viewpoints with our prototype system, we successfully recovered color light field videos for various fast-moving microscopic specimens with a spatial resolution of 0.79 megapixels at 30 frames per second, corresponding to an unprecedented data throughput of 562.5 MB/s for light field microscopy. We also demonstrated the use of the reported platform for different applications, including post-capture refocusing, phase reconstruction, 3D imaging, and optical metrology.
本文提出了一种利用相机阵列进行高分辨率光场显微镜成像的新方法。在这种方法中，我们应用了一个两级中继系统，将显微镜的孔径平面扩展到成像透镜阵列的尺寸，并利用传感器阵列获取由相应成像透镜形成的不同子孔径图像。通过将来自5×5个视点的校正和同步图像与我们的原型系统相结合，我们成功地以每秒30帧的速度恢复了各种快速移动微观标本的彩色光场视频，空间分辨率为0.79兆像素，对应于光场显微镜前所未有的562.5MB/s的数据吞吐量。我们还展示了所报道平台在不同应用中的使用，包括捕获后重新聚焦、相位重建、3D成像和光学计量。
