Pechlivanis Alexandros, Papaioannou Konstantinos G, Tsalis George, Saraslanidis Ploutarchos, Mougios Vassilis, Theodoridis Georgios A
Biomolecular Medicine, Division of Computational and Systems Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London , SW7 2AZ London, United Kingdom.
School of Chemistry, Aristotle University of Thessaloniki , 54124 Thessaloniki, Greece.
J Proteome Res. 2015 Nov 6;14(11):4610-22. doi: 10.1021/acs.jproteome.5b00470. Epub 2015 Oct 12.
The delineation of exercise biochemistry by utilizing metabolic fingerprinting has become an established strategy. We present a combined RP-UPLC-MS and (1)H NMR strategy, supplemented by photometric assays, to monitor the response of the human urinary metabolome to short maximal exercise. Seventeen male volunteers performed two identical sprint sessions on separate days, consisting of three 80 m maximal runs. Using univariate and multivariate analyses, we followed the fluctuation of 37 metabolites at 1, 1.5, and 2 h postexercise. 2-Hydroxyisovalerate, 2-hydroxybutyrate, 2-oxoisocaproate, 3-methyl-2-oxovalerate, 3-hydroxyisobutyrate, 2-oxoisovalerate, 3-hydroxybutyrate, 2-hydroxyisobutyrate, alanine, pyruvate, and fumarate increased 1 h postexercise and then returned toward baseline. Lactate and acetate were higher than baseline at 1 and 1.5 h. Hypoxanthine and inosine remained above baseline throughout the postexercise period. Urate decreased at 1 h and increased at 1.5 h before returning to baseline. Valine, isoleucine, succinate, citrate, trimethylamine, trimethylamine N-oxide, tyrosine, and formate decreased at 1 h and/or 1.5 h postexercise and then returned to baseline. Creatinine gradually decreased over the sampling period. Glycine, 4-aminohippurate, and hippurate remained below baseline throughout the postexercise period. Our findings show that even one-half minute of maximal exercise elicited major perturbations in human metabolism, several of which persisted for at least 2 h.
利用代谢指纹图谱来描绘运动生物化学已成为一种既定策略。我们提出了一种结合反相超高效液相色谱-质谱联用（RP-UPLC-MS）和核磁共振氢谱（¹H NMR）的策略，并辅以光度测定法，以监测人体尿液代谢组对短时间最大强度运动的反应。17名男性志愿者在不同日期进行了两次相同的短跑训练，每次训练包括三次80米的最大强度跑步。通过单变量和多变量分析，我们跟踪了运动后1小时、1.5小时和2小时37种代谢物的波动情况。2-羟基异戊酸、2-羟基丁酸、2-氧代异己酸、3-甲基-2-氧代戊酸、3-羟基异丁酸、2-氧代异戊酸、3-羟基丁酸、2-羟基异丁酸、丙氨酸、丙酮酸和富马酸在运动后1小时增加，然后恢复到基线水平。乳酸和乙酸在1小时和1.5小时高于基线水平。次黄嘌呤和肌苷在运动后整个时间段都保持在基线水平以上。尿酸在1小时下降，在1.5小时上升，然后恢复到基线水平。缬氨酸、异亮氨酸、琥珀酸、柠檬酸、三甲胺、氧化三甲胺、酪氨酸和甲酸在运动后1小时和/或1.5小时下降，然后恢复到基线水平。肌酐在采样期间逐渐下降。甘氨酸、4-氨基马尿酸和马尿酸在运动后整个时间段都低于基线水平。我们的研究结果表明，即使是半分钟的最大强度运动也会引起人体代谢的重大扰动，其中一些扰动至少持续2小时。
