Zheng Bijun, Xu Zhe, Yang Li, Jiang Guangyang, Chen Jia, Yang Yichen, Tian Yongqiang
College of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu, 610065, People's Republic of China.
Antonie Van Leeuwenhoek. 2023 Dec;116(12):1433-1445. doi: 10.1007/s10482-023-01895-0. Epub 2023 Oct 24.
A Gram-positive, aerobic, rod-shaped non-motile, non-sporulating bacterium, designated CSA2, was isolated from chromium-containing soils collected from a chemical plant. The 16S rRNA gene sequence of strain CSA2 showed the highest homology with Leucobacter chromiireducens subsp. solipictus (97.85%), Leucobacter chromiireducens subsp. chromiireducens (97.85%). The digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI) and the amino acid identity (AAI) values among strains CSA2 and the selected Leucobacter species were 20.6-23.4% (dDDH), 72.67-78.03% (ANI) and 66.39-76.16% (AAI), falling below the recommended thresholds for species delimitation. The principal fatty acids were anteiso-C, iso-C and anteiso-C. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and an unknown glycolipid. The major menaquinones detected were MK-10 and MK-11. The cell-wall amino acids included 2,4-diaminobutyric acid, threonine, glutamic acid, alanine and glycine. Based on molecular feature, phenotypic and chemotaxonomic, strain CSA2 was considered to be a novel species of the genus Leucobacter., and the name Leucobacter edaphi sp. nov. is proposed. The type strain is CSA2 (= JCM 34360 = CGMCC 1.18747).
从某化工厂采集的含铬土壤中分离出一株革兰氏阳性、需氧、杆状、不运动、不产芽孢的细菌，命名为CSA2。菌株CSA2的16S rRNA基因序列与嗜土嗜铬白细胞杆菌亚种（97.85%）、嗜铬白细胞杆菌亚种（97.85%）具有最高同源性。菌株CSA2与所选白细胞杆菌属菌株之间的数字DNA-DNA杂交（dDDH）、平均核苷酸同一性（ANI）和氨基酸同一性（AAI）值分别为20.6 - 23.4%（dDDH）、72.67 - 78.03%（ANI）和66.39 - 76.16%（AAI），均低于物种界定的推荐阈值。主要脂肪酸为anteiso-C、iso-C和anteiso-C。极性脂质为磷脂酰甘油、二磷脂酰甘油和一种未知糖脂。检测到的主要甲基萘醌为MK-10和MK-11。细胞壁氨基酸包括2,4-二氨基丁酸、苏氨酸、谷氨酸、丙氨酸和甘氨酸。基于分子特征、表型和化学分类学，菌株CSA2被认为是白细胞杆菌属的一个新物种，提议命名为嗜土白细胞杆菌（Leucobacter edaphi sp. nov.）。模式菌株为CSA2（=JCM 34360 = CGMCC 1.18747）。
