用于直接从细菌培养物中增强检测碳青霉烯酶产生菌的简化Carba NP试验方案
Simplified Protocol for Carba NP Test for Enhanced Detection of Carbapenemase Producers Directly from Bacterial Cultures.
作者信息
Pasteran Fernando, Tijet Nathalie, Melano Roberto G, Corso Alejandra
机构信息
Servicio Antimicrobianos, Laboratorio Nacional y Regional de Referencia en Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas (INEI), ANLIS Dr. Carlos G. Malbrán, Ministerio de Salud de la Nación, Buenos Aires, Argentina.
Public Health Ontario Laboratories (PHOL), Toronto, Ontario, Canada.
出版信息
J Clin Microbiol. 2015 Dec;53(12):3908-11. doi: 10.1128/JCM.02032-15. Epub 2015 Sep 30.
Abstract
We compared carbapenemase detection among 266 Gram-negative bacilli (161 carbapenemase producers) using the Carba NP tests issued by the CLSI (CNPt-CLSI) and a novel protocol (CNPt-direct) designed for carbapenemase detection direct from bacterial cultures (instead of bacterial extracts required by the CLSI tests). The specificities were comparable (100%), but the CNPt-direct was more sensitive (98% versus 84%). The CNPt-direct was easier to perform due to the direct use of colonies and offered a more robust detection of carbapenemase producers.
摘要
我们使用美国临床和实验室标准协会（CLSI）发布的碳青霉烯酶检测方法（CNPt-CLSI）和一种专为直接从细菌培养物中检测碳青霉烯酶而设计的新方法（CNPt-direct，而非CLSI检测方法所要求的细菌提取物），对266株革兰氏阴性杆菌（161株碳青霉烯酶产生菌）进行了碳青霉烯酶检测比较。两种方法的特异性相当（均为100%），但CNPt-direct更敏感（98%对84%）。由于直接使用菌落，CNPt-direct操作更简便，且对碳青霉烯酶产生菌的检测更可靠。
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2
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