Osterblad Monica, Hakanen Antti J, Jalava Jari
Antimicrobial Resistance Unit, National Institute for Health and Welfare, Turku, Finland
Antimicrobial Resistance Unit, National Institute for Health and Welfare, Turku, Finland Dept. of Medical Microbiology and Immunology, University of Turku, Turku, Finland.
Antimicrob Agents Chemother. 2014 Dec;58(12):7553-6. doi: 10.1128/AAC.02761-13. Epub 2014 Sep 22.
The Carba NP test was evaluated against a panel of 61 carbapenemase-producing bacterial species (15 producing class A carbapenemases, 15 producing class D carbapenemases, and 31 producing metallo-β-lactamases) and against 111 isolates with non-wild-type carbapenem susceptibility but not producing carbapenemase. Carbapenemase production was verified by PCR and UV-spectrophotometric measurement of imipenem hydrolysis. No false positives were seen, but there were consistent problems with the detection of OXA-48-like enzymes and also some rarer class A enzymes.
针对一组61种产碳青霉烯酶的细菌（15种产A类碳青霉烯酶、15种产D类碳青霉烯酶和31种产金属β-内酰胺酶）以及111株碳青霉烯药敏非野生型但不产碳青霉烯酶的菌株，对Carba NP试验进行了评估。通过PCR和亚胺培南水解的紫外分光光度法测定来验证碳青霉烯酶的产生。未发现假阳性，但在检测OXA-48样酶以及一些罕见的A类酶时存在持续问题。
