Yang Bin, Wei Wei, Shi Yunying, Huang Zhuochun, Cai Bei, Zhang Junlong, Ying Binwu, Wang Lanlan
Department of Laboratory Medicine, West China Hospital, Sichuan University, 610041 Chengdu, China.
West China School of Medicine, Sichuan University, 610041 Chengdu, China.
PLoS One. 2015 Oct 1;10(10):e0139554. doi: 10.1371/journal.pone.0139554. eCollection 2015.
MicroRNA 146a (miR-146a) is a 19 to 23 nucleotide long, small non-coding RNA with gene regulatory functions that has influence on the pathogenesis of many diseases. A single nucleotide polymorphism (rs2910164 C>G) in pre-miR-146a is correlated with the expression of miR-146a. The aim of this study was to perform an association analysis of rs2910164 with IgA nephropathy in adult patients from a Chinese Han population.
A total of 145 patients with renal biopsy-proved IgA nephropathy (IgAN) and 179 healthy controls were recruited to the current study. rs2910164 was genotyped by the polymerase chain reaction (PCR) and high-resolution melting methods (HRM). Clinical characteristics and pathology grading of patients with IgAN were recorded at the time of kidney biopsy.
There were significant differences among the population of patients grouped by different age of onset in a co-dominant model (CG vs. CC vs. GG) (p = 0.033) and a recessive model (CG+CC vs. GG) (p = 0.001). However, no significant difference was observed in the distribution of genotypes between cases and controls (p = 0.144). There was also no significant difference between rs2910164 and patient quantitative traits (all p > 0.003) or different pathology grading (Lee's grading system and tubular atrophy/interstitial fibrosis in the Oxford classification) (all p > 0.05).
There was no association of rs2910164 with susceptibility to IgAN in adults from a Chinese Han population. However, rs2910164 was correlated with the age of onset of IgAN in adult patients.
微小RNA 146a（miR-146a）是一种长度为19至23个核苷酸的小型非编码RNA，具有基因调控功能，对多种疾病的发病机制有影响。前体miR-146a中的单核苷酸多态性（rs2910164 C>G）与miR-146a的表达相关。本研究旨在对中国汉族成年患者中rs2910164与IgA肾病进行关联分析。
本研究共纳入145例经肾活检证实的IgA肾病（IgAN）患者和179例健康对照。采用聚合酶链反应（PCR）和高分辨率熔解方法（HRM）对rs2910164进行基因分型。在肾活检时记录IgAN患者的临床特征和病理分级。
在共显性模型（CG vs. CC vs. GG）（p = 0.033）和隐性模型（CG+CC vs. GG）（p = 0.001）中，按不同发病年龄分组的患者人群之间存在显著差异。然而，病例组和对照组之间的基因型分布没有显著差异（p = 0.144）。rs2910164与患者定量性状（所有p > 0.003）或不同病理分级（Lee分级系统和牛津分类中的肾小管萎缩/间质纤维化）之间也没有显著差异（所有p > 0.05）。
在中国汉族成年人中，rs2910164与IgAN易感性无关。然而，rs2910164与成年IgAN患者的发病年龄相关。
