Qu Jing, Song Min, Xie Jian, Huang Xiao-Yu, Hu Xiao-Meng, Gan Rui-Huan, Zhao Yong, Lin Li-Song, Chen Jiang, Lin Xu, Zheng Da-Li, Lu You-Guang
Department of Preventive Dentistry, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
Department of Pathology, Affiliated Stomatological Hospital, Fujian Medical University, Fuzhou, 350002, Fujian, People's Republic of China.
Mol Cell Biochem. 2016 Jan;411(1-2):135-41. doi: 10.1007/s11010-015-2575-z. Epub 2015 Oct 1.
Many studies have explored whether the Notch signaling pathway has a tumor-suppressive or an oncogenic role in various tumors; however, the role of the Notch signaling pathway in salivary adenoid cystic carcinoma (SACC) is still unknown. In this study, we attempt to define the role of Notch2 signaling in cell growth, invasion, and migration in SACC. We compared Notch2 expression in clinical SACC samples with that of normal samples by using immunohistochemical staining. Then, we down-regulated Notch2 expression to observe the effect of Notch2 on proliferation, invasion, migration, and the expression of known target genes of Notch signal pathway. According to our results, Notch2 expression was higher in SACC tissues compared with normal tissues. Knockdown of Notch2 inhibited cell proliferation, invasion, and migration in vitro and down-regulated the expression of HEY2 and CCND1. The results of this study suggest that Notch2 has an essential role in the cell growth, invasion, and migration of SACC. Notch2 may therefore be a potential target gene for the treatment of SACC by interfering with cell growth and metastasis.
许多研究探讨了Notch信号通路在各种肿瘤中是具有肿瘤抑制作用还是致癌作用；然而，Notch信号通路在涎腺腺样囊性癌（SACC）中的作用仍不清楚。在本研究中，我们试图明确Notch2信号在SACC细胞生长、侵袭和迁移中的作用。我们通过免疫组化染色比较了临床SACC样本与正常样本中Notch2的表达。然后，我们下调Notch2表达以观察Notch2对增殖、侵袭、迁移以及Notch信号通路已知靶基因表达的影响。根据我们的结果，与正常组织相比，SACC组织中Notch2表达更高。Notch2基因敲低在体外抑制了细胞增殖、侵袭和迁移，并下调了HEY2和CCND1的表达。本研究结果表明，Notch2在SACC的细胞生长、侵袭和迁移中起重要作用。因此，Notch2可能是通过干扰细胞生长和转移来治疗SACC的潜在靶基因。
