Mahboubi Mohaddese, Kashani Leila Mohammad Taghizadeh
a Department of Microbiology and.
b Department of Traditional Medicine , Medicinal Plants Research Center of Barij , Kashan , Iran.
Pharm Biol. 2016;54(4):720-5. doi: 10.3109/13880209.2015.1072831. Epub 2015 Oct 1.
Commiphora molmol Engl (Burseraceae) or myrrh has been traditionally used for the treatment of skin fungal infections.
This study evaluates the antifungal activity of myrrh ethanol extract and essential oil against skin dermatophytes.
The antifungal evaluations were performed by the food poisoning technique (250 ppm) and micro-broth dilution assay (800-6.25 µg/mL) against Trichophyton rubrum, T. mentagrophytes, Microsporum canis, M. gypseum, and T. verrucosum. The chemical composition of myrrh oil and ethanol extract was analyzed by GC and GC-MS.
Furanoeudesma 1,3-diene and menthofuran were the main components of myrrh oil, while 2-tert-butyl-1,4-naphthoquinone, benzenemethanol,3-methoxy-α-phenyl, and curzerene were the main components of myrrh ethanol extract. The inhibitory effect of myrrh oil and ethanol extract against dermatophytes were 43.1-61.6% and 12.5-27.5%, respectively. The MIC and MFC values of myrrh oil were 25-100 and 25-200 µg/mL while these amounts for ethanol extract were 25-400 and 25-400 µg/mL, respectively. Therefore, myrrh oil had higher antifungal activity than that of the ethanol extract. Both extracts showed good anti-elastase activity.
The results of our investigation confirmed the traditional uses of C. molmol as a poultice for the treatment of cutaneous fungal infections.
没药树（橄榄科）或没药传统上用于治疗皮肤真菌感染。
本研究评估没药乙醇提取物和精油对皮肤癣菌的抗真菌活性。
采用食物中毒技术（250 ppm）和微量肉汤稀释法（800 - 6.25 μg/mL）对红色毛癣菌、须癣毛癣菌、犬小孢子菌、石膏样小孢子菌和疣状毛癣菌进行抗真菌评估。通过气相色谱（GC）和气相色谱 - 质谱联用（GC - MS）分析没药油和乙醇提取物的化学成分。
呋喃桉叶二烯和薄荷呋喃是没药油的主要成分，而2 - 叔丁基 - 1,4 - 萘醌、3 - 甲氧基 - α - 苯基苯甲醇和莪术烯是没药乙醇提取物的主要成分。没药油和乙醇提取物对皮肤癣菌的抑制作用分别为43.1 - 61.6%和12.5 - 27.5%。没药油的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）值分别为25 - 100和25 - 200 μg/mL，而乙醇提取物的这些值分别为25 - 400和25 - 400 μg/mL。因此，没药油的抗真菌活性高于乙醇提取物。两种提取物均显示出良好的抗弹性蛋白酶活性。
我们的研究结果证实了没药树作为治疗皮肤真菌感染的敷剂的传统用途。
