Loskill Peter, Marcus Sivan G, Mathur Anurag, Reese Willie Mae, Healy Kevin E
Department of Bioengineering, University of California at Berkeley, Berkeley, California, United States of America; Department of Materials Science and Engineering, University of California at Berkeley, Berkeley, California, United States of America; California Institute for Quantitative Biosciences (QB3), University of California at Berkeley, Berkeley, California, United States of America.
Department of Bioengineering, University of California at Berkeley, Berkeley, California, United States of America.
PLoS One. 2015 Oct 6;10(10):e0139587. doi: 10.1371/journal.pone.0139587. eCollection 2015.
Human organ-on-a-chip systems for drug screening have evolved as feasible alternatives to animal models, which are unreliable, expensive, and at times erroneous. While chips featuring single organs can be of great use for both pharmaceutical testing and basic organ-level studies, the huge potential of the organ-on-a-chip technology is revealed by connecting multiple organs on one chip to create a single integrated system for sophisticated fundamental biological studies and devising therapies for disease. Furthermore, since most organ-on-a-chip systems require special protocols with organ-specific media for the differentiation and maturation of the tissues, multi-organ systems will need to be temporally customizable and flexible in terms of the time point of connection of the individual organ units. We present a customizable Lego®-like plug & play system, μOrgano, which enables initial individual culture of single organ-on-a-chip systems and subsequent connection to create integrated multi-organ microphysiological systems. As a proof of concept, the μOrgano system was used to connect multiple heart chips in series with excellent cell viability and spontaneously physiological beat rates.
用于药物筛选的人体芯片器官系统已发展成为动物模型的可行替代方案，动物模型不可靠、成本高且有时会出现错误。虽然具有单个器官的芯片对于药物测试和基础器官水平研究都非常有用，但通过将多个器官连接在一个芯片上以创建用于复杂基础生物学研究和疾病治疗设计的单一集成系统，芯片器官技术的巨大潜力得以展现。此外，由于大多数芯片器官系统需要使用特定于器官的培养基的特殊方案来实现组织的分化和成熟，多器官系统在单个器官单元的连接时间点方面需要在时间上可定制且灵活。我们展示了一种类似乐高积木的可定制即插即用系统μOrgano，它能够对单个芯片器官系统进行初始单独培养，并随后进行连接以创建集成的多器官微生理系统。作为概念验证，μOrgano系统被用于串联连接多个心脏芯片，细胞活力良好且自发生理搏动率正常。
