Kuang Dai, Xu Xuebin, Meng Jianghong, Yang Xiaowei, Jin Huiming, Shi Weimin, Pan Haijian, Liao Ming, Su Xudong, Shi Xianmin, Zhang Jianmin
Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.
Infect Genet Evol. 2015 Dec;36:294-299. doi: 10.1016/j.meegid.2015.10.003. Epub 2015 Oct 9.
Salmonella Newport (S. Newport) is a major serotype associated with human salmonellosis. A total of 79 S. Newport recovered from humans and other sources in China were characterized for antimicrobial susceptibility, virulence gene profiles and molecular subtypes using pulsed field gel electrophoresis (PFGE). Approximately 63.3% of the isolates were susceptible to all of 16 antimicrobials tested. Nearly one third of the isolates (31.6%) were resistant to sulfisoxazole, 20.3% to tetracycline and 13.9% to nalidixic acid. Twelve isolates (15.2%) were resistant to three or more antimicrobials. Among 10 virulence genes detected, Salmonella pathogenicity island genes avrA, ssaQ, mgtC, siiD, and sopB and fimbrial gene bcfC were present in most of the isolates (93.7% to 100%). Overall, we observed nine distinct virulence gene profiles, three of which (VP1, VP2 and VP3) were most common (86.1%). A total of 56 PFGE patterns were identified and mainly grouped into seven clusters (A to G) with 80% pattern similarity. Isolates from aquatic product shared a high similarity with those from humans in several clusters, highlighting a potential risk of aquatic product as a source of S. Newport that infect humans. Furthermore, there was a strong association between certain PFGE clusters and virulence gene profiles, suggesting virulence subtyping can be a useful epidemiological tool to discriminate S. Newport isolates.
纽波特沙门氏菌（S. Newport）是一种与人类沙门氏菌病相关的主要血清型。对从中国人类和其他来源分离出的79株纽波特沙门氏菌进行了抗菌药物敏感性、毒力基因谱和分子亚型分析，采用脉冲场凝胶电泳（PFGE）技术。约63.3%的分离株对所检测的16种抗菌药物均敏感。近三分之一的分离株（31.6%）对磺胺异恶唑耐药，20.3%对四环素耐药，13.9%对萘啶酸耐药。12株分离株（15.2%）对三种或更多种抗菌药物耐药。在检测的10个毒力基因中，大多数分离株（93.7%至100%）存在沙门氏菌致病岛基因avrA、ssaQ、mgtC、siiD、sopB和菌毛基因bcfC。总体而言，我们观察到9种不同的毒力基因谱，其中三种（VP1、VP2和VP3）最为常见（86.1%）。共鉴定出56种PFGE图谱，主要分为7个簇（A至G），图谱相似性为80%。来自水产品的分离株在几个簇中与来自人类的分离株具有高度相似性，突出了水产品作为感染人类的纽波特沙门氏菌来源的潜在风险。此外，某些PFGE簇与毒力基因谱之间存在很强的关联，表明毒力亚型分析可以作为区分纽波特沙门氏菌分离株的有用流行病学工具。
