Liu Helin, Fan Yanyan, Wang Jianhai, Song Zhongsen, Shi Hao, Han Rongcheng, Sha Yinlin, Jiang Yuqiang
Single molecule &Nanobiology Laboratory, Department of Biophysics, School of Basic Medical Sciences and Biomed-X Center, Peking University, Beijing 100191, China.
State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Sci Rep. 2015 Oct 8;5:14879. doi: 10.1038/srep14879.
Luminescence thermometry usually suffer from cellular complexity of the biochemical environment (such as pH and ionic strength), and thus the accuracy and reliability of the determined intracellular temperature are directly affected. Herein, a photoluminescent nanothermometer composed of polymer encapsulated quantum dots (P-QD) has been developed. And the prepared nanothermometer exhibits some advantages: such as non-sensitivity to pH and ionic strength, as well as high detection sensitivity and ultrahigh reversibility. The intracellular temperature was accurately determined under physiological conditions with different pH and ionic strength, and direct measurement of thermogenesis in individual cells has been achieved.
发光测温法通常会受到生化环境（如pH值和离子强度）的细胞复杂性影响，因此所测定的细胞内温度的准确性和可靠性会直接受到影响。在此，一种由聚合物包裹量子点（P-QD）组成的光致发光纳米温度计被研发出来。并且所制备的纳米温度计具有一些优点：如对pH值和离子强度不敏感，以及检测灵敏度高和超高可逆性。在不同pH值和离子强度的生理条件下准确测定了细胞内温度，并且实现了对单个细胞内热生成的直接测量。
