Fadaei R, Parvaz E, Emamgholipour S, Moradi N, Vatannejad A, Najafi M, Doosti M
Department of Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Horm Metab Res. 2016 Apr;48(4):269-74. doi: 10.1055/s-0035-1564133. Epub 2015 Oct 14.
It is evident that coronary artery disease (CAD) is closely associated with abnormal glucose and lipid metabolism. Notably, dysregulation of inflammatory pathways and immune system also contribute to CAD development. Recently, it has been suggested that visfatin, a proinflammatory adipocytokine, may be involved in several inflammatory and metabolic diseases. In this study, we evaluated the serum visfatin levels and its mRNA expression in peripheral blood mononuclear cells (PBMCs) from CAD patients compared with control subjects. We also studied the correlation between visfatin gene expression and serum levels with clinical and metabolic parameters. This study was conducted on 56 male patients with CAD confirmed by angiography and 30 healthy men as controls. CAD severity was determined based on the number of vessels. Study of gene expression in PBMCs was performed using real time-PCR, and serum levels of visfatin and 25-hydroxyvitamin D were measured by ELISA. We found that serum visfatin levels and its gene expression in PBMCs were increased in patients with CAD compared with the control group (p=0.027 and p=0.016, respectively). Also, visfatin gene expression was positively correlated with visfatin levels and both these variables had a strong positive correlation with the severity of CAD. It appears that elevated mRNA expression and circulating level of visfatin might be of relevance to the pathogenesis and severity of CAD. However, further studies are necessary to better clarify the associations between visfatin and CAD.
显而易见，冠状动脉疾病（CAD）与糖脂代谢异常密切相关。值得注意的是，炎症通路和免疫系统的失调也促成了CAD的发展。最近，有人提出内脂素，一种促炎性脂肪细胞因子，可能与几种炎症和代谢性疾病有关。在本研究中，我们评估了CAD患者与对照受试者外周血单核细胞（PBMC）中的血清内脂素水平及其mRNA表达。我们还研究了内脂素基因表达和血清水平与临床及代谢参数之间的相关性。本研究对56例经血管造影确诊的男性CAD患者和30例健康男性作为对照进行。CAD的严重程度根据血管数量确定。使用实时PCR进行PBMC中的基因表达研究，并通过ELISA测量血清内脂素和25-羟基维生素D水平。我们发现，与对照组相比，CAD患者的血清内脂素水平及其在PBMC中的基因表达增加（分别为p = 0.027和p = 0.016）。此外，内脂素基因表达与内脂素水平呈正相关，并且这两个变量均与CAD的严重程度呈强正相关。内脂素mRNA表达升高和循环水平升高似乎与CAD的发病机制和严重程度有关。然而，需要进一步研究以更好地阐明内脂素与CAD之间的关联。
