埃博拉病毒糖蛋白通过NPC1+内溶酶体介导融合。
Ebolavirus Glycoprotein Directs Fusion through NPC1+ Endolysosomes.
作者信息
Simmons James A, D'Souza Ryan S, Ruas Margarida, Galione Antony, Casanova James E, White Judith M
机构信息
Department of Cell Biology, University of Virginia, Charlottesville, Virginia, USA.
Department of Pharmacology, University of Oxford, Oxford, United Kingdom.
出版信息
J Virol. 2015 Oct 14;90(1):605-10. doi: 10.1128/JVI.01828-15. Print 2016 Jan 1.
Abstract
Ebolavirus, a deadly hemorrhagic fever virus, was thought to enter cells through endolysosomes harboring its glycoprotein receptor, Niemann-Pick C1. However, an alternate model was recently proposed in which ebolavirus enters through a later NPC1-negative endosome that contains two-pore Ca(2+) channel 2 (TPC2), a newly identified ebolavirus entry factor. Here, using live cell imaging, we obtained evidence that in contrast to the new model, ebolavirus enters cells through endolysosomes that contain both NPC1 and TPC2.
摘要
埃博拉病毒是一种致命的出血热病毒，曾被认为是通过携带其糖蛋白受体尼曼-匹克病C1型（Niemann-Pick C1，NPC1）的内溶酶体进入细胞的。然而，最近有人提出了另一种模型，即埃博拉病毒通过一个后期的NPC1阴性内体进入细胞，该内体含有双孔钙离子通道2（two-pore Ca(2+) channel 2，TPC2），这是一种新发现的埃博拉病毒进入因子。在这里，我们通过活细胞成像获得的证据表明，与新模型相反，埃博拉病毒是通过同时含有NPC1和TPC2的内溶酶体进入细胞的。
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