Srivastava Deepika, Shanker Asheesh
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali, Rajasthan, 304022, India.
Interdiscip Sci. 2016 Dec;8(4):327-336. doi: 10.1007/s12539-015-0129-4. Epub 2015 Oct 15.
Basal angiosperms or Magnoliids is an important clade of commercially important plants which mainly include spices and edible fruits. In this study, 17 chloroplast genome sequences belonging to clade Magnoliids were screened for the identification of chloroplast simple sequence repeats (cpSSRs). Simple sequence repeats or microsatellites are short stretches of DNA up to 1-6 base pair in length. These repeats are ubiquitous and play important role in the development of molecular markers and to study the mapping of traits of economic, medical or ecological interest. A total of 479 SSRs were detected, showing average density of 1 SSR/6.91 kb. Depending on the repeat units, the length of SSRs ranged from 12 to 24 bp for mono-, 12 to 18 bp for di-, 12 to 26 bp for tri-, 12 to 24 bp for tetra-, 15 bp for penta- and 18 bp for hexanucleotide repeats. Mononucleotide repeats were the most frequent (207, 43.21 %) followed by tetranucleotide repeats (130, 27.13 %). Penta- and hexanucleotide repeats were least frequent or absent in these chloroplast genomes.
基部被子植物或木兰类植物是一类具有重要商业价值的植物分支，主要包括香料和可食用水果。在本研究中，筛选了17个属于木兰类植物分支的叶绿体基因组序列，以鉴定叶绿体简单序列重复（cpSSR）。简单序列重复或微卫星是长度达1 - 6个碱基对的短DNA片段。这些重复序列普遍存在，在分子标记的开发以及研究具有经济、医学或生态意义的性状定位方面发挥着重要作用。共检测到479个SSR，平均密度为1个SSR / 6.91 kb。根据重复单元，单核苷酸重复的SSR长度为12至24 bp，二核苷酸重复为12至18 bp，三核苷酸重复为12至26 bp，四核苷酸重复为12至24 bp，五核苷酸重复为15 bp，六核苷酸重复为18 bp。单核苷酸重复最为常见（207个，占43.21%），其次是四核苷酸重复（130个，占27.13%）。在这些叶绿体基因组中，五核苷酸和六核苷酸重复最少见或不存在。
