Humar Matjaž, Gather Malte C, Yun Seok-Hyun
Opt Express. 2015 Oct 19;23(21):27865-79. doi: 10.1364/OE.23.027865.
Biological cell lasers are promising novel building blocks of future biocompatible optical systems and offer new approaches to cellular sensing and cytometry in a microfluidic setting. Here, we demonstrate a simple method for providing optical gain by using a variety of standard fluorescent dyes. The dye gain medium can be located inside or outside a cell, or in both, which gives flexibility in experimental design and makes the method applicable to all cell types. Due to the higher refractive index of the cytoplasm compared to the surrounding medium, a cell acts as a convex lens in a planar Fabry-Perot cavity. Its effect on the stability of the laser cavity is analyzed and utilized to suppress lasing outside cells. The resonance modes depend on the shape and internal structure of the cell. As proof of concept, we show how the laser output modes are affected by the osmotic pressure.
生物细胞激光器是未来生物相容性光学系统中很有前景的新型构建模块，并为微流体环境中的细胞传感和细胞计数提供了新方法。在此，我们展示了一种使用多种标准荧光染料来提供光学增益的简单方法。染料增益介质可以位于细胞内部、外部或两者之中，这为实验设计提供了灵活性，并使该方法适用于所有细胞类型。由于细胞质的折射率高于周围介质，细胞在平面法布里 - 珀罗腔中充当凸透镜。分析了其对激光腔稳定性的影响，并利用这一点抑制细胞外的激光发射。共振模式取决于细胞的形状和内部结构。作为概念验证，我们展示了激光输出模式是如何受到渗透压影响的。
