• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

大肠杆菌中表达绿色荧光蛋白的基因编程激光。

Lasing from Escherichia coli bacteria genetically programmed to express green fluorescent protein.

机构信息

Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital 50 Blossom St, BAR-8, Boston, Massachusetts 02114, USA.

出版信息

Opt Lett. 2011 Aug 15;36(16):3299-301. doi: 10.1364/ol.36.003299.

DOI:10.1364/ol.36.003299
PMID:21847240
Abstract

We report on lasing action from colonies of Escherichia coli bacteria that are genetically programmed to synthesize the green fluorescent protein (GFP). When embedded in a Fabry-Perot type cavity and excited by ns-pulses of blue light (465 nm), the bacteria generate green laser emission (∼520 nm). Broad illumination of pump light yields simultaneous lasing over a large area in bacterial colonies.

摘要

我们报告了大肠杆菌(Escherichia coli)菌落的激光作用,这些大肠杆菌经过基因编程合成了绿色荧光蛋白(GFP)。当它们被嵌入法布里-珀罗(Fabry-Perot)型腔中,并通过纳秒级的蓝光脉冲(465nm)激发时,细菌会产生绿色激光发射(~520nm)。宽波段的泵浦光照射会在细菌菌落中产生大面积的同时激光。

相似文献

1
Lasing from Escherichia coli bacteria genetically programmed to express green fluorescent protein.大肠杆菌中表达绿色荧光蛋白的基因编程激光。
Opt Lett. 2011 Aug 15;36(16):3299-301. doi: 10.1364/ol.36.003299.
2
Screening of recombinant Escherichia coli using activation of green fluorescent protein as an indicator.以绿色荧光蛋白激活作为指标筛选重组大肠杆菌。
Biochem Biophys Res Commun. 2014 Sep 12;452(1):32-5. doi: 10.1016/j.bbrc.2014.08.038. Epub 2014 Aug 17.
3
Use of genetically engineered Escherichia coli to monitor ingestion, loss, and transfer of bacteria in termites.利用基因工程大肠杆菌监测白蚁体内细菌的摄取、损失和转移。
Curr Microbiol. 2005 Feb;50(2):119-23. doi: 10.1007/s00284-004-4428-y. Epub 2005 Feb 3.
4
Enhanced random lasing in ZnO nanocombs assisted by Fabry-Perot resonance.法布里-珀罗共振辅助的ZnO纳米梳中的增强随机激光
Opt Express. 2011 Apr 25;19(9):8728-34. doi: 10.1364/OE.19.008728.
5
Green fluorescent protein for detection of the probiotic microorganism Escherichia coli strain Nissle 1917 (EcN) in vivo.用于在体内检测益生菌微生物大肠杆菌Nissle 1917菌株(EcN)的绿色荧光蛋白。
J Microbiol Methods. 2005 Jun;61(3):389-98. doi: 10.1016/j.mimet.2005.01.007.
6
Characterization of a 3.3-kb plasmid of Escherichia coli O157:H7 and evaluation of stability of genetically engineered derivatives of this plasmid expressing green fluorescence.大肠杆菌O157:H7 3.3-kb质粒的特性分析以及该表达绿色荧光的质粒基因工程衍生物的稳定性评估。
Vet Microbiol. 2008 Dec 10;132(3-4):421-7. doi: 10.1016/j.vetmic.2008.05.016. Epub 2008 May 24.
7
Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments.利用绿色荧光蛋白监测基因工程菌在水生环境中的存活情况。
Appl Environ Microbiol. 1996 Sep;62(9):3486-8. doi: 10.1128/aem.62.9.3486-3488.1996.
8
DPSS yellow-green 561-nm lasers for improved fluorochrome detection by flow cytometry.用于通过流式细胞术改善荧光染料检测的二极管泵浦固体(DPSS)561纳米黄绿激光器。
Cytometry A. 2005 Nov;68(1):36-44. doi: 10.1002/cyto.a.20182.
9
Fluorescent reference strains of bacteria by chromosomal integration of a modified green fluorescent protein gene.通过修饰的绿色荧光蛋白基因的染色体整合构建细菌荧光参考菌株。
Appl Microbiol Biotechnol. 2008 Jan;77(6):1287-95. doi: 10.1007/s00253-007-1253-9. Epub 2007 Nov 9.
10
[Characteristics of atrazine-degrading genetically engineered microorganism (GEM) labeled with green fluorescent protein (GFP)].
Huan Jing Ke Xue. 2006 Jul;27(7):1439-43.

引用本文的文献

1
Monitoring Various Bioactivities at the Molecular, Cellular, Tissue, and Organism Levels via Biological Lasers.通过生物激光器在分子、细胞、组织和机体水平上监测各种生物活性。
Sensors (Basel). 2022 Apr 20;22(9):3149. doi: 10.3390/s22093149.
2
Polarization Angle Dependence of Optical Gain in a Hybrid Structure of Alexa-Flour 488/M13 Bacteriophage.Alexa荧光488/ M13噬菌体混合结构中光学增益的偏振角依赖性
Nanomaterials (Basel). 2021 Dec 6;11(12):3309. doi: 10.3390/nano11123309.
3
Biophotonic probes for bio-detection and imaging.用于生物检测和成像的生物光子探针。
Light Sci Appl. 2021 Jun 9;10(1):124. doi: 10.1038/s41377-021-00561-2.
4
Review of biosensing with whispering-gallery mode lasers.回音壁模式激光生物传感综述。
Light Sci Appl. 2021 Feb 26;10(1):42. doi: 10.1038/s41377-021-00471-3.
5
Enhanced Biophotocurrent Generation in Living Photosynthetic Optical Resonator.活体质光合光学谐振器中增强的生物光电流产生
Adv Sci (Weinh). 2020 Apr 19;7(11):1903707. doi: 10.1002/advs.201903707. eCollection 2020 Jun.
6
Virus lasers for biological detection.病毒激光器用于生物探测。
Nat Commun. 2019 Aug 9;10(1):3594. doi: 10.1038/s41467-019-11604-z.
7
Whispering-gallery-mode emission from biological luminescent protein microcavity assemblies.生物发光蛋白微腔组件的回音壁模式发射
Optica. 2017;4(2):222-228. doi: 10.1364/OPTICA.4.000222. Epub 2017 Feb 13.
8
Versatile tissue lasers based on high-Q Fabry-Pérot microcavities.基于高品质因子法布里-珀罗微腔的多功能组织激光器。
Lab Chip. 2017 Jan 31;17(3):538-548. doi: 10.1039/c6lc01457g.
9
Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling.利用染料和荧光蛋白标记提高全息显微镜中细菌的跟踪和分辨率。
Front Chem. 2016 Apr 19;4:17. doi: 10.3389/fchem.2016.00017. eCollection 2016.
10
Cellular dye lasers: lasing thresholds and sensing in a planar resonator.细胞染料激光器:平面谐振器中的激光阈值与传感
Opt Express. 2015 Oct 19;23(21):27865-79. doi: 10.1364/OE.23.027865.