Maza Johnathan C, Nimmo Zachary M, Young Douglas D
Department of Chemistry, College of William & Mary, Williamsburg, Virginia 23187, USA.
Chem Commun (Camb). 2016 Jan 4;52(1):88-91. doi: 10.1039/c5cc08287k.
The importance of bioconjugates within the field of chemistry drives the need for novel methodologies for their preparation. Well-defined and stable bioconjugates are easily accessible via the utilization of unnatural amino acids (UAAs). As such, we have synthesized and incorporated two new UAAs into green fluorescent protein, and optimized a novel Cadiot-Chodkiewicz bioconjugation, effectively expanding the toolbox of chemical reactions that can be employed in the preparation of bioconjugates.
生物共轭物在化学领域的重要性推动了对其制备新方法的需求。通过使用非天然氨基酸（UAA），可以轻松获得定义明确且稳定的生物共轭物。因此，我们已经合成了两种新的非天然氨基酸并将其整合到绿色荧光蛋白中，同时优化了一种新型的卡迪奥 - 乔德凯维茨生物共轭反应，有效地扩展了可用于制备生物共轭物的化学反应工具箱。
