Rahaman Md Habibur, Islam Tarequl, Colwell Rita R, Alam Munirul
Department of Biology and Chemistry, North South University, Dhaka Bangladesh.
Enteric and Food Microbiology Lab, Center for Communicable Diseases, International Center for Diarrheal Disease Research, Dhaka Bangladesh.
Front Microbiol. 2015 Oct 6;6:1040. doi: 10.3389/fmicb.2015.01040. eCollection 2015.
Vibrio cholerae, the etiological agent of cholera, has been a scourge for centuries. Cholera remains a serious health threat for developing countries and has been responsible for millions of deaths globally over the past 200 years. Identification of V. cholerae has been accomplished using a variety of methods, ranging from phenotypic strategies to DNA based molecular typing and currently whole genomic approaches. This array of methods has been adopted in epidemiological investigations, either singly or in the aggregate, and more recently for evolutionary analyses of V. cholerae. Because the new technologies have been developed at an ever increasing pace, this review of the range of fingerprinting strategies, their relative advantages and limitations, and cholera case studies was undertaken. The task was challenging, considering the vast amount of the information available. To assist the study, key references representative of several areas of research are provided with the intent to provide readers with a comprehensive view of recent advances in the molecular epidemiology of V. cholerae. Suggestions for ways to obviate many of the current limitations of typing techniques are also provided. In summary, a comparative report has been prepared that includes the range from traditional typing to whole genomic strategies.
霍乱弧菌是霍乱的病原体，数百年来一直是一大祸害。霍乱仍然对发展中国家的健康构成严重威胁，在过去200年里已在全球造成数百万人死亡。霍乱弧菌的鉴定已通过多种方法完成，从表型策略到基于DNA的分子分型，目前还有全基因组方法。这一系列方法已被单独或综合用于流行病学调查，最近还用于霍乱弧菌的进化分析。由于新技术的发展速度不断加快，因此对一系列指纹识别策略、它们的相对优势和局限性以及霍乱病例研究进行了综述。考虑到可用信息的数量巨大，这项任务具有挑战性。为辅助该研究，提供了代表几个研究领域的关键参考文献，旨在为读者提供霍乱弧菌分子流行病学最新进展的全面观点。还提供了消除当前分型技术许多局限性的方法建议。总之，已编写了一份比较报告，涵盖从传统分型到全基因组策略的范围。
