Doblas A, Roche E, Ampudia-Blasco F J, Martínez-Corral M, Saavedra G, Garcia-Sucerquia J
3D Imaging and Display Laboratory, Department of Optics, University of Valencia, E-46100 Burjassot, Spain.
Department of Applied Biology-Nutrition, Institute of Bioengineering, University of Miguel Hernandez, E-03203 Elche, Spain.
J Microsc. 2016 Mar;261(3):285-90. doi: 10.1111/jmi.12331. Epub 2015 Oct 26.
Diabetes is currently the world's fastest growing chronic disease and it is caused by deficient production of insulin by the endocrine pancreas or by abnormal insulin action in peripheral tissues. This results in persistent hyperglycaemia that over time may produce chronic diabetic complications. Determination of glycated haemoglobin level is currently the gold standard method to evaluate and control sustained hyperglycaemia in diabetic people. This measurement is currently made by high-performance liquid chromatography, which is a complex chemical process that requires the extraction of blood from the antecubital vein. To reduce the complexity of that measurement, we propose a fully-optical technique that is based in the fact that there are changes in the optical properties of erythrocytes due to the presence of glucose-derived adducts in the haemoglobin molecule. To evaluate these changes, we propose to perform quantitative phase maps of erythrocytes by using telecentric digital holographic microscopy. Our experiments show that telecentric digital holographic microscopy allows detecting, almost in real time and from a single drop of blood, significant differences between erythrocytes of diabetic patients and healthy patients. Besides, our phase measurements are well correlated with the values of glycated haemoglobin and the blood glucose values.
糖尿病是目前全球增长最快的慢性病，它由内分泌胰腺胰岛素分泌不足或外周组织胰岛素作用异常引起。这会导致持续性高血糖，随着时间推移可能引发慢性糖尿病并发症。糖化血红蛋白水平的测定是目前评估和控制糖尿病患者持续性高血糖的金标准方法。目前这种测量是通过高效液相色谱法进行的，这是一个复杂的化学过程，需要从前臂静脉采血。为了降低该测量的复杂性，我们提出一种全光学技术，其基于这样一个事实：由于血红蛋白分子中存在葡萄糖衍生的加合物，红细胞的光学特性会发生变化。为了评估这些变化，我们建议使用远心数字全息显微镜对红细胞进行定量相图分析。我们的实验表明，远心数字全息显微镜几乎可以实时且从一滴血中检测出糖尿病患者和健康患者红细胞之间的显著差异。此外，我们的相位测量结果与糖化血红蛋白值和血糖值具有良好的相关性。
