Jojima Toru, Inui Masayuki
a Research Institute of Innovative Technology for the Earth ; Kizugawa , Kyoto , Japan.
Bioengineered. 2015;6(6):328-34. doi: 10.1080/21655979.2015.1111493.
The glycolytic pathway is a main driving force in the fermentation process as it produces energy, cell component precursors, and fermentation products. Given its importance, the glycolytic pathway can be considered as an attractive target for the metabolic engineering of industrial microorganisms. However, many attempts to enhance glycolytic flux, by overexpressing homologous or heterologous genes encoding glycolytic enzymes, have been unsuccessful. In contrast, significant enhancement in glycolytic flux has been observed in studies with bacteria, specifically, Corynebacterium glutamicum. Although there has been a recent increase in the number of successful applications of this technology, little is known about the mechanisms leading to the enhancement of glycolytic flux. To explore the rational applications of glycolytic pathway engineering in biocatalyst development, this review summarizes recent successful studies as well as past attempts.
糖酵解途径是发酵过程中的主要驱动力，因为它能产生能量、细胞成分前体和发酵产物。鉴于其重要性，糖酵解途径可被视为工业微生物代谢工程的一个有吸引力的目标。然而，许多通过过表达编码糖酵解酶的同源或异源基因来增强糖酵解通量的尝试都未成功。相比之下，在对细菌（特别是谷氨酸棒杆菌）的研究中观察到糖酵解通量有显著增强。尽管这项技术最近成功应用的数量有所增加，但对于导致糖酵解通量增强的机制却知之甚少。为了探索糖酵解途径工程在生物催化剂开发中的合理应用，本综述总结了近期的成功研究以及过去的尝试。
