Herranz-Diez C, Li Q, Lamprecht C, Mas-Moruno C, Neubauer S, Kessler H, Manero J M, Guillem-Martí J, Selhuber-Unkel C
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Technical University of Catalonia (UPC), Spain.
Dept. Biocompatible Nanomaterials, Institute for Materials Science, University of Kiel, Kaiserstr. 2, D-24143 Kiel, Germany.
Colloids Surf B Biointerfaces. 2015 Dec 1;136:704-11. doi: 10.1016/j.colsurfb.2015.10.008. Epub 2015 Oct 20.
Implant materials require optimal biointegration, including strong and stable cell-material interactions from the early stages of implantation. Ti-based alloys with low elastic modulus are attracting a lot of interest for avoiding stress shielding, but their osseointegration potential is still very low. In this study, we report on how cell adhesion is influenced by linear RGD, cyclic RGD, and recombinant fibronectin fragment III8-10 coated on titanium versus a novel low-modulus TiNbHf alloy. The bioactive molecules were either physisorbed or covalently coupled to the substrates and their conformation on the surfaces was investigated with atomic force microscopy (AFM). The influence of the different bioactive coatings on the adhesion of rat mesenchymal stem cells was evaluated using cell culture assays and quantitatively analyzed at the single cell level by AFM-based single-cell force spectroscopy. Our results show that bioactive moieties, particularly fibronectin fragment III8-10, improve cell adhesion on titanium and TiNbHf and that the covalent tethering of such molecules provides the most promising strategy to biofunctionalize these materials. Therefore, the use of recombinant protein fragments is of high importance for improving the osseointegration potential of implant materials.
植入材料需要最佳的生物整合，包括从植入早期就具有强大而稳定的细胞-材料相互作用。低弹性模量的钛基合金因可避免应力屏蔽而备受关注，但其骨整合潜力仍然很低。在本研究中，我们报告了线性RGD、环状RGD和重组纤连蛋白片段III8-10包被在钛及新型低模量TiNbHf合金上时对细胞黏附的影响。生物活性分子通过物理吸附或共价偶联到基底上，并利用原子力显微镜（AFM）研究其在表面的构象。使用细胞培养试验评估不同生物活性涂层对大鼠间充质干细胞黏附的影响，并通过基于AFM的单细胞力谱在单细胞水平进行定量分析。我们的结果表明，生物活性部分，特别是纤连蛋白片段III8-10，可改善细胞在钛和TiNbHf上的黏附，并且此类分子的共价连接为这些材料的生物功能化提供了最有前景的策略。因此，重组蛋白片段的使用对于提高植入材料的骨整合潜力至关重要。
