Murakami Hiroshi, Shimomura Yumi, Matsumoto Mitsuharu, Lane Geoffrey J, Yamataka Atsuyuki, Okawada Manabu
Department of Pediatric General and Urogenital Surgery, Juntendo University School of Medicine, Tokyo, Japan.
Research Laboratories, Kyodo Milk Industry Co., Ltd, Tokyo, Japan.
Pediatr Surg Int. 2016 Jan;32(1):37-43. doi: 10.1007/s00383-015-3810-0. Epub 2015 Oct 29.
To assess the impact of urgent surgery on neonates and the value of an orally administered probiotic preparation of Bifidobacterium animalis subsp. lactis LKM512 (LKM) using fecal DNA sequencing to analyze intestinal microbiota.
Subjects for this study were 13 neonates born at our institution. Surgical cases required surgery within 3 days of birth. Groups studied were surgical cases administered LKM (n = 4; LKM+), surgical cases not administered have surgery and were not administered LKM (n = 2; CS), and normal healthy neonates (n = 3; CN). Stool specimens (20 mg) were collected five times (after birth, and on days 3, 7, 10, and 14 after surgery in surgical cases, and after birth, and on days 4, 8, 11, and 15 of life in controls).
Clinical data were similar for LKM+ and LKM-. Enterobacteriaceae, Streptococcaceae, Staphylococcaceae and Bifidobacteriaceae were identified in the descending order of abundance in CS stool. Streptococcaceae, Staphylococcaceae, Enterococcaceae and Bifidobacteriaceae were identified in the descending order of abundance in LKM+ stool. Bifidobacteriaceae, Enterobacteriaceae, Staphylococcaceae and Streptococcaceae were identified in the descending order of abundance in LKM- stool. Unexpectedly, Bifidobacteriaceae was significantly more abundant in LKM- than LKM+ (p < 0.05).
Surgical stress appears to affect intestinal microbiota considerably. Probiotic administration in neonates requires clarification.
评估急诊手术对新生儿的影响以及口服动物双歧杆菌乳酸亚种LKM512（LKM）益生菌制剂的价值，使用粪便DNA测序分析肠道微生物群。
本研究的受试者为在我院出生的13例新生儿。手术病例要求在出生后3天内进行手术。研究组包括接受LKM治疗的手术病例（n = 4；LKM+）、未接受LKM治疗的手术病例（n = 2；CS）和正常健康新生儿（n = 3；CN）。收集五次粪便标本（20毫克）（手术病例在出生后、手术后第3、7、10和14天，对照组在出生后、生命第4、8、11和15天）。
LKM+组和LKM-组的临床数据相似。在CS组粪便中，肠杆菌科、链球菌科、葡萄球菌科和双歧杆菌科按丰度从高到低排序。在LKM+组粪便中，链球菌科、葡萄球菌科、肠球菌科和双歧杆菌科按丰度从高到低排序。在LKM-组粪便中，双歧杆菌科、肠杆菌科、葡萄球菌科和链球菌科按丰度从高到低排序。出乎意料的是，LKM-组中的双歧杆菌科比LKM+组显著丰富（p < 0.05）。
手术应激似乎对肠道微生物群有相当大的影响。新生儿益生菌给药需要进一步明确。
