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与外源不饱和脂肪酸补充相关的，经高压均质处理的巴氏酵母细胞膜脂肪酸变化及去饱和酶表达

Cell membrane fatty acid changes and desaturase expression of Saccharomyces bayanus exposed to high pressure homogenization in relation to the supplementation of exogenous unsaturated fatty acids.

作者信息

Serrazanetti Diana I, Patrignani Francesca, Russo Alessandra, Vannini Lucia, Siroli Lorenzo, Gardini Fausto, Lanciotti Rosalba

机构信息

Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Università degli Studi di Bologna Cesena, Italy.

Dipartimento di Scienze e Tecnologie Agro-alimentari, Università degli Studi di Bologna Cesena, Italy.

出版信息

Front Microbiol. 2015 Oct 12;6:1105. doi: 10.3389/fmicb.2015.01105. eCollection 2015.

DOI:10.3389/fmicb.2015.01105
PMID:26528258
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4600958/
Abstract

AIMS

The aim of this work was to study the responses of Saccharomyces bayanus cells exposed to sub-lethal high-pressure homogenization (HPH) and determine whether the plasmatic membrane can sense HPH in the presence, or absence, of exogenous unsaturated fatty acids (UFAs) in the growth medium.

METHODS AND RESULTS

High-pressure homogenization damaged and caused the collapse of cell walls and membranes of a portion of cells; however, HPH did not significantly affect S. bayanus cell viability (less than 0.3 Log CFU ml(-1)). HPH strongly affected the membrane fatty acid (FA) composition by increasing the percentage of total UFA when compared with saturated fatty acids. The gene expression showed that the transcription of OLE1, ERG3, and ERG11 increased after HPH. The presence of exogenous UFA abolished HPH-induced effects on the OLE1 and ERG3 genes, increased the percentage of membrane lipids and decreased the expression of OLE1 and ERG3 within 30 min of treatment.

CONCLUSION

The results suggest a key role for UFA in the microbial cell response to sub-lethal stress. In addition, these data provide insight into the molecular basis of the response of S. bayanus to this innovative technology.

SIGNIFICANCE AND IMPACT OF THE STUDY

Elucidation of the mechanism of action for sub-lethal HPH will enable the utilization of this technology to modulate the starter performance at the industrial scale.

摘要

目的

本研究旨在探究巴氏酵母细胞在经受亚致死高压均质处理（HPH）后的反应，并确定在生长培养基中存在或不存在外源不饱和脂肪酸（UFA）的情况下，质膜是否能感知HPH。

方法与结果

高压均质处理会破坏并导致部分细胞的细胞壁和细胞膜坍塌；然而，HPH对巴氏酵母细胞活力的影响并不显著（降低幅度小于0.3 Log CFU ml⁻¹）。与饱和脂肪酸相比，HPH通过增加总UFA的百分比，强烈影响了膜脂肪酸（FA）组成。基因表达显示，HPH处理后OLE1、ERG3和ERG11的转录增加。外源UFA的存在消除了HPH对OLE1和ERG3基因的诱导作用，在处理30分钟内增加了膜脂百分比并降低了OLE1和ERG3的表达。

结论

结果表明UFA在微生物细胞对亚致死应激的反应中起关键作用。此外，这些数据为巴氏酵母对这项创新技术的反应分子基础提供了见解。

研究的意义与影响

阐明亚致死HPH的作用机制将有助于在工业规模上利用该技术调节发酵剂性能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/32487a7ca686/fmicb-06-01105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/8e2ab47c3709/fmicb-06-01105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/ff5b296cd35b/fmicb-06-01105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/c9c9c53d8618/fmicb-06-01105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/32487a7ca686/fmicb-06-01105-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/8e2ab47c3709/fmicb-06-01105-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/ff5b296cd35b/fmicb-06-01105-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/c9c9c53d8618/fmicb-06-01105-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ad0/4600958/32487a7ca686/fmicb-06-01105-g004.jpg

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