Sbaghdi Thania, Garneau Julian R, Yersin Simon, Chaucheyras-Durand Frédérique, Bocquet Michel, Moné Anne, El Alaoui Hicham, Bulet Philippe, Blot Nicolas, Delbac Frédéric
Laboratoire "Microorganismes: Génome et Environnement", CNRS, Université Clermont Auvergne, F-63000 Clermont-Ferrand, France.
Department of Fundamental Microbiology, University of Lausanne, Campus UNIL-Sorge, 1015 Lausanne, Switzerland.
Microorganisms. 2024 Jan 18;12(1):192. doi: 10.3390/microorganisms12010192.
The honey bee is exposed to a variety of biotic and abiotic stressors, such as the highly prevalent microsporidian parasite ( and neonicotinoid insecticides. Both can affect honey bee physiology and microbial gut communities, eventually reducing its lifespan. They can also have a combined effect on the insect's survival. The use of bacterial probiotics has been proposed to improve honey bee health, but their beneficial effect remains an open question. In the present study, western honey bees were experimentally infected with spores, chronically exposed to the neonicotinoid thiamethoxam, and/or supplied daily with the homofermentative bacterium MA18/5M thought to improve the honey bees' tolerance to the parasite. Deep shotgun metagenomic sequencing allowed the response of the gut microbiota to be investigated with a taxonomic resolution at the species level. All treatments induced significant changes in honey bee gut bacterial communities. infection increased the abundance of , , and and reduced the abundance of , , and spp. Supplementation with overturned some of these alterations, bringing back the abundance of some altered species close to the relative abundance found in the controls. Surprisingly, the exposure to thiamethoxam also restored the relative abundance of some species modulated by . This study shows that stressors and probiotics may have an antagonistic impact on honey bee gut bacterial communities and that may have a protective effect against the dysbiosis induced by an infection with
蜜蜂面临着各种生物和非生物应激源，例如高度普遍的微孢子虫寄生虫（以及新烟碱类杀虫剂）。这两者都会影响蜜蜂的生理机能和肠道微生物群落，最终缩短其寿命。它们还可能对蜜蜂的生存产生综合影响。有人提出使用细菌益生菌来改善蜜蜂健康，但它们的有益效果仍是一个悬而未决的问题。在本研究中，对西方蜜蜂进行实验性感染孢子，长期暴露于新烟碱类噻虫嗪中，和/或每天提供被认为能提高蜜蜂对寄生虫耐受性的同型发酵细菌MA18/5M。深度鸟枪法宏基因组测序能够在物种水平上以分类分辨率研究肠道微生物群的反应。所有处理都引起了蜜蜂肠道细菌群落的显著变化。感染增加了[具体物种1]、[具体物种2]和[具体物种3]的丰度，并降低了[具体物种4]、[具体物种5]和[具体物种6]属的丰度。补充MA18/5M扭转了其中一些变化，使一些改变物种的丰度恢复到接近对照组中发现的相对丰度。令人惊讶的是，暴露于噻虫嗪也恢复了一些受[感染因素]调节的物种的相对丰度。这项研究表明，应激源和益生菌可能对蜜蜂肠道细菌群落产生拮抗作用，并且MA18/5M可能对由[感染因素]感染引起的生态失调具有保护作用
