Huang Zhaobin, Li Guizhen, Lai Qiliang, Gu Li, Shao Zongze
Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR, China.
State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen 361005, PR, China.
Int J Syst Evol Microbiol. 2016 Feb;66(2):587-591. doi: 10.1099/ijsem.0.000755. Epub 2015 Nov 9.
A Gram-stain-negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain Lxc1, was isolated from the surface of a marine red alga, sp., which was collected from the coastal regions in Jinjiang, Fujian Province, China. Colonies of the strain were orange-yellow, circular and smooth. The 16S rRNA gene of strain Lxc1 had maximum sequence similarity with E4-9a (97.1 %), followed by PAMC 27137, DJ3, AKS293 and AKS 432 (similarities < 96.4 %). Phylogenetic analysis showed strain Lxc1 formed a tight cluster with E4-9a and DJ3, but represented a novel lineage belonging to the genus . The predominant fatty acids were iso-C G (18.3 %), iso-C (16.7 %), iso-C 3-OH (10.6 %) and iso-C 3-OH (8.6 %). Menaquinone-6 (MK-6) was the only respiratory quinone present. The DNA G+C content of strain Lxc1 was 31.7 mol%. Combining the results above, it was ascertained that strain Lxc1 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Lxc1 ( = MCCC 1A01567 = KCTC 42808).
从中国福建省晋江沿海地区采集的海洋红藻表面分离出一株革兰氏阴性、需氧、无鞭毛的杆状细菌,命名为Lxc1菌株。该菌株的菌落为橙黄色,圆形且光滑。Lxc1菌株的16S rRNA基因与E4-9a的序列相似性最高(97.1%),其次是PAMC 27137、DJ3、AKS293和AKS 432(相似性<96.4%)。系统发育分析表明,Lxc1菌株与E4-9a和DJ3形成紧密聚类,但代表了一个属于该属的新谱系。主要脂肪酸为异-C15:0(18.3%)、异-C16:0(16.7%)、异-C17:0 3-OH(10.6%)和异-C19:0 3-OH(8.6%)。甲基萘醌-6(MK-6)是唯一存在的呼吸醌。Lxc1菌株的DNA G+C含量为31.7 mol%。综合上述结果,确定Lxc1菌株代表该属的一个新物种,为此提出新物种名称[具体属名] sp. nov.。模式菌株为Lxc1(=MCCC 1A01567 = KCTC 42808)。