• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蜡样芽孢杆菌I型β-内酰胺酶基因的分子克隆及核苷酸序列

Molecular cloning and nucleotide sequence of the type I beta-lactamase gene from Bacillus cereus.

作者信息

Sloma A, Gross M

出版信息

Nucleic Acids Res. 1983 Jul 25;11(14):4997-5004. doi: 10.1093/nar/11.14.4997.

DOI:10.1093/nar/11.14.4997
PMID:6308567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326101/
Abstract

The gene for the type I beta-lactamase from Bacillus cereus has been cloned in Bacillus subtilis and Escherichia coli. In B. subtilis, penicillinase activity is detected and the enzyme is secreted. In E. coli, the gene confers ampicillin resistance. The cloned insert is 4.3 kb in length and DNA sequencing has revealed the location of the gene, its promoter and signal peptide.

摘要

蜡样芽孢杆菌I型β-内酰胺酶基因已在枯草芽孢杆菌和大肠杆菌中克隆。在枯草芽孢杆菌中，可检测到青霉素酶活性，且该酶会被分泌。在大肠杆菌中，该基因赋予氨苄青霉素抗性。克隆的插入片段长度为4.3 kb，DNA测序已揭示了该基因、其启动子和信号肽的位置。

相似文献

1
Molecular cloning and nucleotide sequence of the type I beta-lactamase gene from Bacillus cereus.蜡样芽孢杆菌I型β-内酰胺酶基因的分子克隆及核苷酸序列
Nucleic Acids Res. 1983 Jul 25;11(14):4997-5004. doi: 10.1093/nar/11.14.4997.
2
Cloning and sequencing of the beta-lactamase I gene of Bacillus cereus 5/B and its expression in Bacillus subtilis.蜡样芽孢杆菌5/B的β-内酰胺酶I基因的克隆、测序及其在枯草芽孢杆菌中的表达。
J Bacteriol. 1985 Aug;163(2):487-92. doi: 10.1128/jb.163.2.487-492.1985.
3
Cloning and sequencing of the blaZ gene encoding beta-lactamase III, a lipoprotein of Bacillus cereus 569/H.编码β-内酰胺酶III（蜡样芽孢杆菌569/H的一种脂蛋白）的blaZ基因的克隆与测序
J Bacteriol. 1987 Feb;169(2):579-86. doi: 10.1128/jb.169.2.579-586.1987.
4
The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170.嗜碱芽孢杆菌菌株170的脂青霉素酶基因的核苷酸序列。
Arch Microbiol. 1989;151(2):91-4. doi: 10.1007/BF00414419.
5
Molecular cloning and expression of Bacillus licheniformis beta-lactamase gene in Escherichia coli and Bacillus subtilis.地衣芽孢杆菌β-内酰胺酶基因在大肠杆菌和枯草芽孢杆菌中的分子克隆与表达
J Bacteriol. 1981 Jan;145(1):422-8. doi: 10.1128/jb.145.1.422-428.1981.
6
Processing of Bacillus cereus 569/H beta-lactamase I in Escherichia coli and Bacillus subtilis.蜡样芽孢杆菌569/Hβ-内酰胺酶I在大肠杆菌和枯草芽孢杆菌中的加工处理
J Biol Chem. 1985 Jan 25;260(2):1218-23.
7
Sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (CfiA) from Bacteroides fragilis TAL2480 reveals strong similarity between CfiA and Bacillus cereus beta-lactamase II.对脆弱拟杆菌TAL2480中一种亚胺培南-头孢西丁水解酶（CfiA）的基因进行测序，结果显示CfiA与蜡样芽孢杆菌β-内酰胺酶II有很强的相似性。
J Bacteriol. 1990 May;172(5):2584-93. doi: 10.1128/jb.172.5.2584-2593.1990.
8
Cloning of the genes for penicillinase, penP and penI, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis.地衣芽孢杆菌青霉素酶基因penP和penI在某些载体质粒中的克隆及其在大肠杆菌、枯草芽孢杆菌和地衣芽孢杆菌中的表达。
J Bacteriol. 1981 Sep;147(3):776-86. doi: 10.1128/jb.147.3.776-786.1981.
9
Cloning and sequencing of the metallothioprotein beta-lactamase II gene of Bacillus cereus 569/H in Escherichia coli.蜡样芽孢杆菌569/H金属硫蛋白β-内酰胺酶II基因在大肠杆菌中的克隆与测序
J Bacteriol. 1985 Oct;164(1):223-9. doi: 10.1128/jb.164.1.223-229.1985.
10
Secretion of Escherichia coli beta-lactamase from Bacillus subtilis by the aid of alpha-amylase signal sequence.借助α-淀粉酶信号序列从枯草芽孢杆菌中分泌大肠杆菌β-内酰胺酶。
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5582-6. doi: 10.1073/pnas.79.18.5582.

引用本文的文献

1
Genetic and biochemical analysis of a novel Ambler class A beta-lactamase responsible for cefoxitin resistance in Bacteroides species.对一种新型Ambler A类β-内酰胺酶的遗传和生化分析，该酶导致拟杆菌属对头孢西丁耐药。
Antimicrob Agents Chemother. 1993 May;37(5):1028-36. doi: 10.1128/AAC.37.5.1028.
2
Protein secretion in Bacillus species.芽孢杆菌属中的蛋白质分泌
Microbiol Rev. 1993 Mar;57(1):109-37. doi: 10.1128/mr.57.1.109-137.1993.
3
Cloning and characterization of the endogenous cephalosporinase gene, cepA, from Bacteroides fragilis reveals a new subgroup of Ambler class A beta-lactamases.来自脆弱拟杆菌的内源性头孢菌素酶基因cepA的克隆与特性分析揭示了安布勒A类β-内酰胺酶的一个新亚组。
Antimicrob Agents Chemother. 1993 Nov;37(11):2391-400. doi: 10.1128/AAC.37.11.2391.
4
Compilation of published signal sequences.已发表信号序列的汇编。
Nucleic Acids Res. 1984 Jul 11;12(13):5145-64. doi: 10.1093/nar/12.13.5145.
5
The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.枯草芽孢杆菌分泌型β-葡聚糖酶的基因及遗传控制位点的DNA序列。
Nucleic Acids Res. 1984 Jul 11;12(13):5355-67. doi: 10.1093/nar/12.13.5355.
6
Cloning and sequencing of the metallothioprotein beta-lactamase II gene of Bacillus cereus 569/H in Escherichia coli.蜡样芽孢杆菌569/H金属硫蛋白β-内酰胺酶II基因在大肠杆菌中的克隆与测序
J Bacteriol. 1985 Oct;164(1):223-9. doi: 10.1128/jb.164.1.223-229.1985.
7
Analysis of signals for secretion in the staphylococcal protein A gene.葡萄球菌蛋白A基因分泌信号分析
EMBO J. 1985 Dec 30;4(13B):3901-6. doi: 10.1002/j.1460-2075.1985.tb04164.x.
8
Structural and kinetic studies on beta-lactamase K1 from Klebsiella aerogenes.产气克雷伯菌β-内酰胺酶K1的结构与动力学研究
Biochem J. 1986 Mar 1;234(2):343-7. doi: 10.1042/bj2340343.
9
The guanine and cytosine content of genomic DNA and bacterial evolution.基因组DNA的鸟嘌呤和胞嘧啶含量与细菌进化
Proc Natl Acad Sci U S A. 1987 Jan;84(1):166-9. doi: 10.1073/pnas.84.1.166.
10
The amino acid sequence of a gonococcal growth inhibitor from Staphylococcus haemolyticus.来自溶血葡萄球菌的淋球菌生长抑制剂的氨基酸序列。
Biochem J. 1988 May 15;252(1):87-93. doi: 10.1042/bj2520087.

本文引用的文献

1
Purification of penicillin-induced penicillinase of Bacillus cereus NRRL 569: a comparison of its properties with those of a similarly purified penicillinase produced spontaneously by a constitutive mutant strain.蜡样芽孢杆菌NRRL 569青霉素诱导型青霉素酶的纯化：将其特性与组成型突变株自发产生的类似纯化青霉素酶的特性进行比较。
Biochem J. 1956 Mar;62(3):391-401. doi: 10.1042/bj0620391.
2
Membrane-bound penicillinases in Gram-positive bacteria.革兰氏阳性菌中的膜结合青霉素酶。
J Biol Chem. 1982 Apr 25;257(8):4490-5.
3
Direct selection of recombinant plasmids in Bacillus subtilis.枯草芽孢杆菌中重组质粒的直接筛选
Gene. 1982 Dec;20(3):459-69. doi: 10.1016/0378-1119(82)90215-3.
4
Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.地衣芽孢杆菌青霉素酶：基因的核苷酸序列及其对革兰氏阳性菌中分泌蛋白生物合成的影响。
Nucleic Acids Res. 1981 Jun 11;9(11):2577-88. doi: 10.1093/nar/9.11.2577.
5
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
6
Nucleotide sequence of the amylase gene from Bacillus subtilis.枯草芽孢杆菌淀粉酶基因的核苷酸序列。
Nucleic Acids Res. 1983 Jan 25;11(2):237-49. doi: 10.1093/nar/11.2.237.
7
Cloning and expression of a Bacillus coagulans amylase gene in Escherichia coli.凝结芽孢杆菌淀粉酶基因在大肠杆菌中的克隆与表达
Mol Gen Genet. 1982;186(4):507-11. doi: 10.1007/BF00337957.
8
Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the alpha-amylase gene from Bacillus amyloliquefaciens.解淀粉芽孢杆菌α-淀粉酶基因启动子及NH2-末端信号肽区域的核苷酸序列
Gene. 1981 Oct;15(1):43-51. doi: 10.1016/0378-1119(81)90103-7.
9
Fate of transforming DNA following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex.感受态枯草芽孢杆菌摄取转化DNA后的命运。I. 供体-受体复合物的形成及特性
J Mol Biol. 1971 Mar 14;56(2):209-21. doi: 10.1016/0022-2836(71)90460-8.
10
A complementation analysis of the restriction and modification of DNA in Escherichia coli.大肠杆菌中DNA限制与修饰的互补分析。
J Mol Biol. 1969 May 14;41(3):459-72. doi: 10.1016/0022-2836(69)90288-5.