蜡样芽孢杆菌I型β-内酰胺酶基因的分子克隆及核苷酸序列
Molecular cloning and nucleotide sequence of the type I beta-lactamase gene from Bacillus cereus.
作者信息
Sloma A, Gross M
出版信息
Nucleic Acids Res. 1983 Jul 25;11(14):4997-5004. doi: 10.1093/nar/11.14.4997.
Abstract
The gene for the type I beta-lactamase from Bacillus cereus has been cloned in Bacillus subtilis and Escherichia coli. In B. subtilis, penicillinase activity is detected and the enzyme is secreted. In E. coli, the gene confers ampicillin resistance. The cloned insert is 4.3 kb in length and DNA sequencing has revealed the location of the gene, its promoter and signal peptide.
摘要
蜡样芽孢杆菌I型β-内酰胺酶基因已在枯草芽孢杆菌和大肠杆菌中克隆。在枯草芽孢杆菌中，可检测到青霉素酶活性，且该酶会被分泌。在大肠杆菌中，该基因赋予氨苄青霉素抗性。克隆的插入片段长度为4.3 kb，DNA测序已揭示了该基因、其启动子和信号肽的位置。
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