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濒危的加拉帕戈斯特有白斑沙鲈(Paralabrax albomaculatus)10个多态微卫星位点的分离与特征分析

Isolation and characterization of 10 polymorphic microsatellite loci for the endangered Galapagos-endemic whitespotted sandbass (Paralabrax albomaculatus).

作者信息

Bertolotti Alicia C, Griffiths Sarah M, Truelove Nathan K, Box Stephen J, Preziosi Richard F, Salinas de Leon Pelayo

机构信息

Faculty of Life Sciences, The University of Manchester , Manchester , United Kingdom.

Smithsonian Marine Station, Smithsonian , Fort Pierce Florida , United States.

出版信息

PeerJ. 2015 Oct 6;3:e1253. doi: 10.7717/peerj.1253. eCollection 2015.

Abstract

The white-spotted sandbass (Paralabrax albomaculatus) is a commercially important species in the Galapagos Marine Reserve, but is classified as endangered in the IUCN Red List. For this study, 10 microsatellite loci were isolated and characterized using Illumina paired-end sequencing. These loci can be used for genetic studies of population structure and connectivity to aid in the management of the white-spotted sandbass and other closely-related species. The 10 characterized loci were polymorphic, with 11-49 alleles per locus, and observed heterozygosity ranged from 0.575 to 0.964. This set of markers is the first to be developed for this species.

摘要

白斑沙鲈(Paralabrax albomaculatus)是加拉帕戈斯海洋保护区具有重要商业价值的物种,但在世界自然保护联盟红色名录中被列为濒危物种。在本研究中,利用Illumina双端测序分离并鉴定了10个微卫星位点。这些位点可用于种群结构和连通性的遗传研究,以协助管理白斑沙鲈及其他近缘物种。所鉴定的这10个位点具有多态性,每个位点有11 - 49个等位基因,观察到的杂合度范围为0.575至0.964。这组标记是首次针对该物种开发的。

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