Serinkan Cinemre F B, Cinemre Hakan, Karacaer Cengiz, Aydemir Birsen, Nalbant Ahmet, Kaya Tezcan, Tamer Ali
Department of Biochemistry, Sakarya Üniversitesi Tıp Fakültesi Dekanlığı, Sakarya University School of Medicine, Korucuk Kampüsü, Konuralp Bulvarı No:81/1, 54187, Sakarya, Turkey.
Department of Internal Medicine, Sakarya University School of Medicine, Sakarya, Turkey.
Inflamm Res. 2016 Feb;65(2):143-50. doi: 10.1007/s00011-015-0898-6. Epub 2015 Nov 13.
The growth factor midkine (MK) is a protein that is involved in cancer, inflammation, immunity. Vitamin D is a potent immunomodulator. Anti-Saccharomyces cerevisiae antibody (ASCA) is reported in autoimmune disorders, some of which are among the causes of vitamin D deficiency. The objective of this study was to investigate a possible association of MK and ASCA with vitamin D deficiency.
208 adults presented to internal medicine outpatient clinic for history and physical examination has been studied. Serum biochemistry, vitamin D, MK, ASCA-IgG and -IgA, IL-1β, IL-6, IL-8, TNF-α, PDGF, VEGF were obtained.
Vitamin D deficiency was 74.2%. Serum MK level was significantly higher in vitamin D-deficient compared to vitamin D-sufficient individuals (1138.1 ± 262.8 vs 958.6 ± 189 pg/mL, respectively; P < 0.009). Serum MK levels were also significantly higher in both ASCA-IgG and -IgA positives compared to negatives (1318.5 ± 160.3 vs 1065.5 ± 256.1, P = 0.008 and 1347.7 ± 229.7 vs 1070.1 ± 250.9 pg/mL, P = 0.011, respectively). Vitamin D was significantly lower in ASCA positives (P = 0.044).Vitamin D showed positive correlation with IL-1β (r 0.338, P < 0.009) and negative correlation with VEGF (r -0.366, P < 0.004).
MK was significantly elevated in vitamin D deficiency and associated with ASCA positivity which was significantly increased in vitamin D deficiency. These findings suggested that molecular mechanism of vitamin D deficiency may be related with some inflammatory processes.
生长因子中期因子（MK）是一种参与癌症、炎症和免疫的蛋白质。维生素D是一种有效的免疫调节剂。抗酿酒酵母抗体（ASCA）在自身免疫性疾病中被报道，其中一些是维生素D缺乏的原因。本研究的目的是调查MK和ASCA与维生素D缺乏之间可能存在的关联。
对208名到内科门诊进行病史和体格检查的成年人进行了研究。检测了血清生化指标、维生素D、MK、ASCA-IgG和-IgA、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、肿瘤坏死因子-α（TNF-α）、血小板衍生生长因子（PDGF）、血管内皮生长因子（VEGF）。
维生素D缺乏率为74.2%。与维生素D充足的个体相比，维生素D缺乏个体的血清MK水平显著更高（分别为1138.1±262.8与958.6±189 pg/mL；P<0.009）。与ASCA-IgG和-IgA阴性者相比，阳性者的血清MK水平也显著更高（分别为1318.5±160.3与1065.5±256.1，P=0.008；以及1347.7±229.7与1070.1±250.9 pg/mL，P=0.011）。ASCA阳性者的维生素D水平显著更低（P=0.044）。维生素D与IL-1β呈正相关（r=0.338，P<0.009），与VEGF呈负相关（r=-0.366，P<0.004）。
MK在维生素D缺乏时显著升高，且与ASCA阳性相关，而ASCA阳性在维生素D缺乏时显著增加。这些发现提示维生素D缺乏的分子机制可能与某些炎症过程有关。
