Sumazaki R, Motz M, Wolf H, Heinig J, Jilg W, Deinhardt F
Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Federal Republic of Germany.
J Med Virol. 1989 Apr;27(4):304-8. doi: 10.1002/jmv.1890270409.
A new assay was developed for the detection of hepatitis B virus (HBV) in human serum using amplification of a short viral DNA sequence by means of the polymerase chain reaction. As little as 0.4 fg viral DNA, corresponding to about 130 genome equivalents, per ml serum could be detected after the amplification procedure. This assay detected viral DNA in a number of patients with proven or suspected chronic HBV infection who were all negative for HBV DNA in the conventional hybridisation assay. We found HBV DNA in all of six HBeAg-positive and in three of eight HBeAg-negative HBsAg carriers, as well as in all of 11 patients with chronic liver disease with antibodies against the HBV core antigen (anti-HBc) as the sole marker for HBV infection, and in three of five apparently healthy individuals showing only anti HBc. Thus, this method is an important improvement for the diagnosis of persistent HBV infections, especially in patients where a definitive serological diagnosis is not possible.
一种新的检测方法被开发出来，用于通过聚合酶链反应扩增短病毒DNA序列来检测人血清中的乙型肝炎病毒（HBV）。在扩增程序后，每毫升血清中低至0.4 fg的病毒DNA（相当于约130个基因组当量）都能被检测到。该检测方法在一些经证实或疑似慢性HBV感染的患者中检测到了病毒DNA，而这些患者在传统杂交检测中HBV DNA均为阴性。我们在6例HBeAg阳性和8例HBeAg阴性的HBsAg携带者中的3例中发现了HBV DNA，在11例以抗HBV核心抗原抗体（抗-HBc）作为HBV感染唯一标志物的慢性肝病患者中全部发现了HBV DNA，在5例仅显示抗HBc的明显健康个体中的3例中也发现了HBV DNA。因此，这种方法对于持续性HBV感染的诊断是一项重要的改进，特别是对于那些无法进行明确血清学诊断的患者。
