Kanagavel Murugesan, Princy Margreat Alphonse Asirvatham, Arunkumar Manivel, Prabhakaran Shanmugarajan Gnanasekaran, Shanmughapriya Santhanam, Natarajaseenivasan Kalimuthusamy
Medical Microbiology Laboratory, Department of Microbiology, Centre of Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Medical Microbiology Laboratory, Department of Microbiology, Centre of Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Infect Genet Evol. 2016 Jan;37:123-8. doi: 10.1016/j.meegid.2015.11.008. Epub 2015 Nov 11.
Here the rodent carrier status for the transmission of human leptospirosis in Tiruchirappalli, district, Tamil Nadu, India was assessed. The predominantly circulating leptospiral STs were recognized by multilocus sequence typing (MLST). A total of 113 rodents were trapped from different provinces of the Tiruchirappalli district. The most prevalent rodent was Bandicota bengalensis (37.2%), and of the total, 52.2% (n=59) rodents were found to be positive for leptospiral 16S rRNA. These results were validated with a leptospiral culture positivity of 45.8% (n=27). Three isolates from Chennai (2 rodents and 1 human) and 1 human isolate from Tiruchirappalli were included to understand the spatial variations and to track the source of human leptospirosis. The serogroup, serovar, and species level identification of all 31 isolates identified 28 to be Leptospira borgpetersenii serovar Javanica and three as Leptospira interrogans serovar Autumnalis. MLST analysis defined all isolates to the existing ST profiles (ST145 and ST27) with the exception of 6 L. borgpetersenii (ST DR) isolates that showed variations in the sucA and pfkB loci. The DR ST was locally confined to Chatram province of Tiruchirappalli suggesting an epidemiological link. The predominant STs, ST145 and ST-DR form a group, indicating the presence of original strain that subsequently diverged evolutionarily into two STs. The variations between L. borgpetersenii in sucA and pfkB loci may be an indication that evolutionary changes transpired in Tiruchirappalli.
在此，对印度泰米尔纳德邦蒂鲁吉拉伯利地区人类钩端螺旋体病传播的啮齿动物携带者状况进行了评估。通过多位点序列分型（MLST）识别出主要流行的钩端螺旋体序列类型（STs）。从蒂鲁吉拉伯利地区的不同区域共捕获了113只啮齿动物。最常见的啮齿动物是板齿鼠（37.2%），在所有捕获的啮齿动物中，52.2%（n = 59）被发现钩端螺旋体16S rRNA呈阳性。这些结果通过45.8%（n = 27）的钩端螺旋体培养阳性率得到了验证。纳入了来自金奈的3株分离株（2只啮齿动物和1名人类）以及来自蒂鲁吉拉伯利的1株人类分离株，以了解空间差异并追踪人类钩端螺旋体病的来源。对所有31株分离株进行血清群、血清型和种水平鉴定，确定28株为波摩那群爪哇型钩端螺旋体，3株为秋季群问号钩端螺旋体。MLST分析将所有分离株定义为现有的ST谱型（ST145和ST27），但有6株波摩那群钩端螺旋体（ST DR）分离株在sucA和pfkB基因座显示出变异。DR ST在当地局限于蒂鲁吉拉伯利的查特拉姆区域，表明存在流行病学联系。主要的STs，即ST145和ST - DR形成一个组，表明存在原始菌株，该菌株随后在进化上分化为两个STs。波摩那群钩端螺旋体在sucA和pfkB基因座的变异可能表明在蒂鲁吉拉伯利发生了进化变化。
