Kyeong San, Jeong Cheolhwan, Kang Homan, Cho Hong-Jun, Park Sung-Jun, Yang Jin-Kyoung, Kim Sehoon, Kim Hyung-Mo, Jun Bong-Hyun, Lee Yoon-Sik
School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea.
Nano Systems Institute and Interdisciplinary Program in Nano-Science and Technology, Seoul National University, Seoul, Korea.
PLoS One. 2015 Nov 24;10(11):e0143727. doi: 10.1371/journal.pone.0143727. eCollection 2015.
Superparamagnetic Fe3O4 nanoparticles (NPs) based nanomaterials have been exploited in various biotechnology fields including biomolecule separation. However, slow accumulation of Fe3O4 NPs by magnets may limit broad applications of Fe3O4 NP-based nanomaterials. In this study, we report fabrication of Fe3O4 NPs double-layered silica nanoparticles (DL MNPs) with a silica core and highly packed Fe3O4 NPs layers. The DL MNPs had a superparamagnetic property and efficient accumulation kinetics under an external magnetic field. Moreover, the magnetic field-exposed DL MNPs show quantitative accumulation, whereas Fe3O4 NPs single-layered silica nanoparticles (SL MNPs) and silica-coated Fe3O4 NPs produced a saturated plateau under full recovery of the NPs. DL MNPs are promising nanomaterials with great potential to separate and analyze biomolecules.
基于超顺磁性四氧化三铁纳米颗粒（NPs）的纳米材料已被应用于包括生物分子分离在内的各种生物技术领域。然而，磁体对四氧化三铁纳米颗粒的缓慢积累可能会限制基于四氧化三铁纳米颗粒的纳米材料的广泛应用。在本研究中，我们报道了具有二氧化硅核心和高度密集四氧化三铁纳米颗粒层的四氧化三铁纳米颗粒双层二氧化硅纳米颗粒（DL MNPs）的制备。DL MNPs具有超顺磁性，并且在外部磁场下具有高效的积累动力学。此外，暴露于磁场的DL MNPs显示出定量积累，而四氧化三铁纳米颗粒单层二氧化硅纳米颗粒（SL MNPs）和二氧化硅包覆的四氧化三铁纳米颗粒在纳米颗粒完全回收时产生饱和平台。DL MNPs是有前景的纳米材料，在生物分子分离和分析方面具有巨大潜力。
