Li Fan, Cao Lei, Hang Donghua, Wang Fang, Wang Qiugen
Department of Trauma and Orthopedics, Trauma Emergency Center, Shanghai First People's Hospital, Shanghai Jiao Tong University Shanghai 200011, China.
Int J Clin Exp Pathol. 2015 Sep 1;8(9):11414-20. eCollection 2015.
Long non-coding RNAs (lncRNAs) have been shown to play key roles in cancer development and progression. In this study, we focused on lncRNA HOTTIP and investigated its expression pattern, clinical significance, and biological function in osteosarcoma (OS). In the present study, lncRNA HOTTIP expression in OS tissues was examined and its correlation with clinicopathological features and patient prognosis was analyzed. In vitro assays were performed to understand the biological roles of lncRNA HOTTIP in OS progression. In the study, we found that HOTTIP expression was up-regulated in OS tissues, and correlated with advanced clinical stage and distant metastasis. OS patients with high HOTTIP expression level had poorer overall survival than those with low HOTTIP expression. Multivariable Cox proportional hazards regression analysis suggested that increased HOTTIP expression was an independent prognostic factor of overall survival in OS patients. Moreover, the results of in vitro assays showed that the suppression of HOTTIP in OS cells significantly reduced cell proliferation, migration and invasion ability. Our study demonstrated that lncRNA HOTTIP play critical roles in OS progression and could represent a novel prognostic marker and potential therapeutic target in OS patients.
长链非编码RNA（lncRNAs）已被证明在癌症发生和发展中起关键作用。在本研究中，我们聚焦于lncRNA HOTTIP，并研究了其在骨肉瘤（OS）中的表达模式、临床意义及生物学功能。在本研究中，检测了OS组织中lncRNA HOTTIP的表达，并分析了其与临床病理特征及患者预后的相关性。进行了体外实验以了解lncRNA HOTTIP在OS进展中的生物学作用。在该研究中，我们发现HOTTIP在OS组织中表达上调，且与临床晚期和远处转移相关。HOTTIP表达水平高的OS患者总生存期比表达水平低的患者更差。多变量Cox比例风险回归分析表明，HOTTIP表达增加是OS患者总生存期的独立预后因素。此外，体外实验结果显示，抑制OS细胞中的HOTTIP可显著降低细胞增殖、迁移和侵袭能力。我们的研究表明，lncRNA HOTTIP在OS进展中起关键作用，可能是OS患者的一种新型预后标志物和潜在治疗靶点。
