Salazar-Lugo R, Vargas A, Rojas L, Lemus M
Departamento de Bioanálisis, Laboratorio de Proteínas e Inmunotoxicidad, Postgrado de Biología Aplicada, Escuela de Ciencias, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.
Laboratorio de Histología, Instituto de Investigaciones en Biomedicina y Ciencias Aplicadas (IIBCA-UDO), Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela.
Open Vet J. 2013;3(2):145-50. Epub 2013 Dec 25.
We evaluated the effect of cadmium (Cd) on the structure and function of the head kidney in the freshwater fish Colossoma macropomum (C. macropomum). Juveniles were exposed to 0.1 mg/L CdCl2 for 31 days. Blood samples were examined using hematological tests and head kidney histology was determined by light microscopy. The concentration of Cd in the head and trunk kidneys was measured using an atomic absorption spectrophotometer. Cd produced histopathological changes in the head kidney, the most evident of these being: the thickening of the vein wall, an increase in the number of basophils/mast cells close to blood vessels and a severe depletion of hematopoietic precursors especially the granulopoietic series. In the blood, a decrease in the total leucocytes and hemoglobin concentration was observed. Cd-exposed fish showed higher Cd concentrations in the trunk kidney than the head kidney. In conclusion, exposure to Cd affected precursor hematopoietic cells in C. macropomum.
我们评估了镉（Cd）对淡水鱼巨脂鲤（Colossoma macropomum，简称C. macropomum）头部肾脏结构和功能的影响。将幼鱼暴露于0.1 mg/L的CdCl2中31天。通过血液学检测检查血样，并通过光学显微镜确定头部肾脏组织学。使用原子吸收分光光度计测量头部和躯干肾脏中的镉浓度。镉在头部肾脏中产生了组织病理学变化，其中最明显的是：静脉壁增厚、靠近血管的嗜碱性粒细胞/肥大细胞数量增加以及造血前体细胞尤其是粒细胞系的严重耗竭。在血液中，观察到总白细胞和血红蛋白浓度降低。暴露于镉的鱼躯干肾脏中的镉浓度高于头部肾脏。总之，暴露于镉会影响巨脂鲤的造血前体细胞。
