Undergratuate in Biological Sciences, Federal University of Santa Catarina -UFSC, St. Agronomic Engineer Andrei Cristian Ferreira, s/n - Trindade, Florianópolis, SC, 88040-900, Brazil.
Postgraduate Program in Aquaculture, Nilton Lins University, Laranjeiras Park, Professor Nilton Lins Avenue, 3259 - Flores, Manaus, AM, 69058-030, Brazil; Laboratory of Ecophysiology and Molecular Evolution (LEEM), Brazilian National Institute for Research of the Amazon (INPA), André Araújo Avenue, 2.936 - Petrópolis, Manaus, AM, 69067-375, Brazil.
Chemosphere. 2021 Mar;266:128957. doi: 10.1016/j.chemosphere.2020.128957. Epub 2020 Nov 12.
The contamination of aquatic environments by transition metals can have a direct influence on the reproductive process of several organisms in the aquatic biota. This study aimed to evaluate the effect of cadmium and copper on the sperm of tambaqui (Colossoma macropomum). Male (n = 4) and female (n = 4) specimens of C. macropomum were induced to spermiation and ovulation, with sperm being activated in the following media: 0; 0.6; 1.2 and 1.8 mg/L of cadmium (CdCl) and 0; 0.4; 0.8 and 1.2 mg/L of copper (CuCl). Sperm quality was assessed through time (s) and motility rate (%), superoxide dismutase (SOD) and glutathione S-transferase (GST) activities, lipoperoxidation levels (LPO), and morphological characteristics. In parallel, the effects of these metals on the rate of fertilization and hatching of the oocytes were evaluated. The duration and motility rate of sperm were longer in the control treatment, 85.67 ± 11.01 s; 90 ± 0.01%, and progressively decreased to 44.67 ± 4.16 s and 60 ± 5%, respectively, in concentrations of 1.8 mg/L (44.67 ± 4.16 s; 60 ± 5%) of CdCl and to 65.67 ± 3.30 s; 70 ± 5%, respectively, in concentrations of 0.8 mg/L of CuCl. We observed an increase in the activity of the SOD enzyme in sperm cells exposed to 1.2 mg/L of CdCl. The LPO levels were increased significantly in sperm cells exposed to 1.2 and 1.8 mg/L of CdCl and 0.8 mg/L of CuCl. Fertilization and hatching were severely impaired in the presence of Cd and Cu. These data indicate that environments contaminated with cadmium and copper harm the gametes of C. macropomum.
过渡金属对水生环境的污染会直接影响水生生物群的几种生物的生殖过程。本研究旨在评估镉和铜对淡水鳕鱼（Colossoma macropomum）精子的影响。雄性（n=4）和雌性（n=4）淡水鳕鱼被诱导排精和排卵，精子在以下介质中被激活：0；0.6；1.2 和 1.8 mg/L 的镉（CdCl）和 0；0.4；0.8 和 1.2 mg/L 的铜（CuCl）。通过时间（s）和运动率（%）、超氧化物歧化酶（SOD）和谷胱甘肽 S-转移酶（GST）活性、脂质过氧化水平（LPO）和形态特征评估精子质量。同时，评估这些金属对卵子受精率和孵化率的影响。在对照处理中，精子的持续时间和运动率更长，分别为 85.67±11.01 s；90±0.01%，并逐渐降低至 1.8 mg/L（44.67±4.16 s；60±5%）CdCl 的浓度和 0.8 mg/L 的 CuCl 的浓度分别为 65.67±3.30 s；70±5%。我们观察到暴露于 1.2 mg/L CdCl 的精子细胞中 SOD 酶的活性增加。暴露于 1.2 和 1.8 mg/L CdCl 和 0.8 mg/L CuCl 的精子细胞中的 LPO 水平显著增加。存在 Cd 和 Cu 时，受精和孵化受到严重损害。这些数据表明，受镉和铜污染的环境会损害淡水鳕鱼的配子。
Animals (Basel). 2024-12-18
Toxics. 2022-10-19
Zotero 插件