Rai Narendra Prakash, Anekar Jayaprasad, Shivaraja Shankara Y M, Divakar Darshan Devang, Al Kheraif Abdulaziz Abdullah, Ramakrishnaiah Ravikumar, Sebastian Roopa, Raj A C, Al-Hazmi Ali, Mustafa Shabil Mohamed
Department of Oral Medicine and Radiology, Lincoln's University College, Kuala Lumpur, Malaysia E-mail :
Asian Pac J Cancer Prev. 2015;16(17):7497-500. doi: 10.7314/apjcp.2015.16.17.7497.
Tumor markers, designated as a broad group of substances produced by malignancies, could be in the form of biochemical substances, immunological substances, cell surface changes and genetic alterations. Cancer, a disorder of cellular behavior is characterized by alteration of serum glycoproteins. L-fucose, a hexose, which is the terminal sugar in most of the plasma glycoproteins, may be useful as a tumor marker for the detection, monitoring and prognostic assessment of malignancies. The aim of the study was to ascertain the role of serum fucose as a biomarker for early detection of oral cancer and to compare serum fucose levels in healthy controls, leukoplakia and oral cancer patients.
The study included 60 (100.0%) subjects, who were grouped as 20 (33.3%) control subjects, 20 (33.3%) squamous cell carcinoma patients and 20 (33.3%) leukoplakia patients. Fucose estimation was done using UV-visible spectrophotometry based on the method as adopted by Winzler using cysteine reagent. The results were analyzed statistically using ANOVA with Bonferroni post hoc tests.
Results showed a high significance in serum fucose in oral squamous cell carcinoma (OSCC) and leukoplakia subjects compared to normal controls. There was a gradual increase in the values noted from control to leukoplakia and to squamous cell carcinoma.
Estimation of serum fucose may be a reliable marker and can be used as an effective diagnostic biomarker in oral squamous cell carcinoma patients.
肿瘤标志物是由恶性肿瘤产生的一大类物质，其形式可以是生化物质、免疫物质、细胞表面变化和基因改变。癌症是一种细胞行为紊乱疾病，其特征是血清糖蛋白发生改变。L-岩藻糖是一种己糖，是大多数血浆糖蛋白的末端糖，可能作为检测、监测和评估恶性肿瘤预后的肿瘤标志物。本研究的目的是确定血清岩藻糖作为口腔癌早期检测生物标志物的作用，并比较健康对照者、白斑患者和口腔癌患者的血清岩藻糖水平。
本研究纳入60名（100.0%）受试者，分为20名（33.3%）对照者、20名（33.3%）鳞状细胞癌患者和20名（33.3%）白斑患者。采用Winzler使用半胱氨酸试剂的方法，通过紫外可见分光光度法进行岩藻糖测定。结果采用方差分析和Bonferroni事后检验进行统计学分析。
结果显示，与正常对照相比，口腔鳞状细胞癌（OSCC）和白斑患者血清岩藻糖具有高度显著性。从对照到白斑再到鳞状细胞癌，数值逐渐升高。
血清岩藻糖测定可能是一种可靠的标志物，可作为口腔鳞状细胞癌患者有效的诊断生物标志物。
