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Wrinkled1促进甘蓝型油菜开花并调控油脂积累与膜脂合成之间的脂质稳态。

Wrinkled1 Accelerates Flowering and Regulates Lipid Homeostasis between Oil Accumulation and Membrane Lipid Anabolism in Brassica napus.

作者信息

Li Qing, Shao Jianhua, Tang Shaohua, Shen Qingwen, Wang Tiehu, Chen Wenling, Hong Yueyun

机构信息

National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University Wuhan, China.

出版信息

Front Plant Sci. 2015 Nov 19;6:1015. doi: 10.3389/fpls.2015.01015. eCollection 2015.

DOI:10.3389/fpls.2015.01015
PMID:26635841
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4652056/
Abstract

Wrinkled1 (WRI1) belongs to the APETALA2 transcription factor family; it is unique to plants and is a central regulator of oil synthesis in Arabidopsis. The effects of WRI1 on comprehensive lipid metabolism and plant development were unknown, especially in crop plants. This study found that BnWRI1 in Brassica napus accelerated flowering and enhanced oil accumulation in both seeds and leaves without leading to a visible growth inhibition. BnWRI1 decreased storage carbohydrates and increased soluble sugars to facilitate the carbon flux to lipid anabolism. BnWRI1 is localized to the nucleus and directly binds to the AW-box at proximal upstream regions of genes involved in fatty acid (FA) synthesis and lipid assembly. The overexpression (OE) of BnWRI1 resulted in the up-regulation of genes involved in glycolysis, FA synthesis, lipid assembly, and flowering. Lipid profiling revealed increased galactolipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), and phosphatidylcholine (PC) in the leaves of OE plants, whereas it exhibited a reduced level of the galactolipids DGDG and MGDG and increased levels of PC, phosphatidylethanolamide, and oil [triacylglycerol (TAG)] in the siliques of OE plants during the early seed development stage. These results suggest that BnWRI1 is important for homeostasis among TAG, membrane lipids and sugars, and thus facilitates flowering and oil accumulation in B. napus.

摘要

皱叶1（WRI1）属于APETALA2转录因子家族；它是植物特有的，是拟南芥油脂合成的核心调节因子。WRI1对综合脂质代谢和植物发育的影响尚不清楚，尤其是在农作物中。本研究发现，甘蓝型油菜中的BnWRI1加速开花，并增强种子和叶片中的油脂积累，且不会导致明显的生长抑制。BnWRI1减少储存碳水化合物并增加可溶性糖，以促进碳流向脂质合成代谢。BnWRI1定位于细胞核，并直接与参与脂肪酸（FA）合成和脂质组装的基因近端上游区域的AW-box结合。BnWRI1的过表达（OE）导致参与糖酵解、FA合成、脂质组装和开花的基因上调。脂质分析显示，OE植株叶片中的半乳糖脂单半乳糖基二酰基甘油（MGDG）、二半乳糖基二酰基甘油（DGDG）和磷脂酰胆碱（PC）增加，而在种子发育早期阶段，OE植株角果中的半乳糖脂DGDG和MGDG水平降低，PC、磷脂酰乙醇胺和油脂[三酰基甘油（TAG）]水平增加。这些结果表明，BnWRI1对于TAG、膜脂和糖之间的稳态很重要，从而促进甘蓝型油菜的开花和油脂积累。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/1c389cc5bc67/fpls-06-01015-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/b67d45e6be24/fpls-06-01015-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/e445138c1c26/fpls-06-01015-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/ac7348942829/fpls-06-01015-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/1c389cc5bc67/fpls-06-01015-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/60e8f7f5fcc3/fpls-06-01015-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/a21ca2edb0f7/fpls-06-01015-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/108295f46a45/fpls-06-01015-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/4c392e61b70d/fpls-06-01015-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/e445138c1c26/fpls-06-01015-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/ac7348942829/fpls-06-01015-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f0d/4652056/1c389cc5bc67/fpls-06-01015-g010.jpg

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