Banerjee Barnini, R M Saldanha Dominic, Baliga Srikala
Department of Microbiology, Kasturba Medical College, Manipal.
Department of Microbiology, Kannur Medical College, Anjarakandy, Kannur, Kerala.
Iran J Microbiol. 2015 Feb;7(1):55-61.
Over the last two decades, both the incidence of nosocomial candidaemia and the proportion of blood stream infection due to Candida spp. other than Candida albicans have increased. The aims of this study was to identify different species of Candida and risk factors associated with bloodstream infection and detection of biofilm production.
This study was conducted in an 840 bedded tertiary care hospital, over a period of one year. All blood isolates received from patients during this period were screened for candidemia prospectively. Speciation was carried out by standard microbiological method. Biofilm production detection was done by Brachini et al method.
A total of 80 cases of candidemia were identified. Most important risk factor was placement of vascular access devices in all the age groups. Candida albicans accounted for 22 isolates (27.5%) whereas non-albicans Candida spp. accounted for 58 isolates (72.5%). Biofilm production was found in 31 strains (38.75%). Biofilm production was seen more in non-albicans Candidaspp. (83.87%) especially in C. tropicalis (66.67%, 8 of 12).
Non-albicansspecies of Candida were most frequently recovered in our study. So, the epidemiology of Candida infection is changing. Non-albicans Candida spp have the capacity to produce significant amount of biofilm which may be the cause of their reduced susceptibility to antifungal agents.
在过去二十年中，医院念珠菌血症的发病率以及由白色念珠菌以外的念珠菌属引起的血流感染比例均有所上升。本研究的目的是鉴定念珠菌的不同种类、与血流感染相关的危险因素以及生物膜形成的检测。
本研究在一家拥有840张床位的三级护理医院进行，为期一年。在此期间前瞻性地筛查从患者处获得的所有血液分离株是否存在念珠菌血症。通过标准微生物学方法进行菌种鉴定。生物膜形成检测采用布拉基尼等人的方法。
共鉴定出80例念珠菌血症病例。在所有年龄组中，最重要的危险因素是血管通路装置的放置。白色念珠菌占22株（27.5%），而非白色念珠菌属占58株（72.5%）。在31株（38.75%）中发现有生物膜形成。非白色念珠菌属中生物膜形成更为常见（83.87%），尤其是热带念珠菌（66.67%，12株中有8株）。
在我们的研究中，非白色念珠菌属最常被分离出来。因此，念珠菌感染的流行病学正在发生变化。非白色念珠菌属有能力产生大量生物膜，这可能是它们对抗真菌药物敏感性降低的原因。
