Alworth Leanne C, Berghaus Roy D, Kelly Lisa M, Supakorndej Prasit, Burkman Erica J, Savadelis Molly D, Cooper Tanya L, Salyards Gregory W, Harvey Stephen B, Moorhead Andrew R
University Research Animal Resources, Department of Population Health, University of Georgia, Athens, Georgia, USA.
Department of Population Health, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Comp Med. 2015 Dec;65(6):492-8.
The NIH guidelines for survival bleeding of mice and rats note that using the retroorbital plexus has a greater potential for complications than do other methods of blood collection and that this procedure should be performed on anesthetized animals. Lateral saphenous vein puncture has a low potential for complications and can be performed without anesthesia. Mongolian gerbils (Meriones unguiculatus) are the preferred rodent model for filarial parasite research. To monitor microfilaria counts in the blood, blood sampling from the orbital plexus has been the standard. Our goal was to refine the blood collection technique. To determine whether blood collection from the lateral saphenous vein was a feasible alternative to retroorbital sampling, we compared microfilaria counts in blood samples collected by both methods from 21 gerbils infected with the filarial parasitic worm Brugia pahangi. Lateral saphenous vein counts were equivalent to retroorbital counts at relatively high counts (greater than 50 microfilariae per 20 μL) but were significantly lower than retroorbital counts when microfilarial concentrations were lower. Our results indicate that although retroorbital collection may be preferable when low concentrations of microfilariae need to be enumerated, the lateral saphenous vein is a suitable alternative site for blood sampling to determine microfilaremia and is a feasible refinement that can benefit the wellbeing of gerbils.
美国国立卫生研究院（NIH）关于小鼠和大鼠存活期采血的指南指出，与其他采血方法相比，采用眶后静脉丛采血引发并发症的可能性更大，并且该操作应在麻醉动物身上进行。隐外侧静脉穿刺引发并发症的可能性较低，且无需麻醉即可进行。蒙古沙鼠（长爪沙鼠）是丝虫寄生虫研究的首选啮齿动物模型。为监测血液中的微丝蚴数量，从眶静脉丛采血一直是标准方法。我们的目标是改进采血技术。为确定从隐外侧静脉采血是否是眶后采血的可行替代方法，我们比较了从21只感染了丝虫寄生虫彭亨布鲁线虫的沙鼠身上通过这两种方法采集的血样中的微丝蚴数量。在微丝蚴数量相对较高时（每20微升大于50条微丝蚴），隐外侧静脉采血的微丝蚴数量与眶后采血相当，但当微丝蚴浓度较低时，隐外侧静脉采血的微丝蚴数量显著低于眶后采血。我们的结果表明，尽管在需要计数低浓度微丝蚴时眶后采血可能更可取，但隐外侧静脉是用于确定微丝蚴血症的合适替代采血部位，并且是一种可行的改进方法，可有益于沙鼠的健康。
